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Monoclonal antibody against c-terminus of sheeppox virus k3l protein and its application

A monoclonal antibody, sheep pox virus technology, applied in antiviral immunoglobulin, immunoglobulin, microorganism-based methods, etc.

Active Publication Date: 2020-07-10
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The above-mentioned methods are all established for the nucleic acid or structural protein in the middle and late stages of virus replication, so they can only detect the presence of the virus after the virus infects the host for 12 hours or even longer

Method used

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  • Monoclonal antibody against c-terminus of sheeppox virus k3l protein and its application
  • Monoclonal antibody against c-terminus of sheeppox virus k3l protein and its application
  • Monoclonal antibody against c-terminus of sheeppox virus k3l protein and its application

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Embodiment Construction

[0034] The present invention is illustrated below in conjunction with examples. The following content is only used to illustrate the relevant details of the present invention, and should not be interpreted as limiting the protection scope of the present invention.

[0035] In the following examples:

[0036] 1. Strains and Cells

[0037] The virus strain used in the present invention is the Gulang strain of sheep pox virus, and the Vero cells and SP2 / 0 cells used are preserved by the applicant (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences).

[0038] 2. Main reagents

[0039]The pUC57 cloning vector, Top10 competent cells, plasmid extraction kit, XbaⅠ and BamHI restriction enzymes, and T4 DNA ligase were purchased from Dalian Bao Biological Engineering Co., Ltd.; DMEM medium, RPMI-1640 cell culture medium and fetal bovine Serum was purchased from Gibco Company; gel recovery kit was purchased from AXYGEN Company; pET11d expression vector, BL...

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Abstract

The invention discloses a hybridoma cell line capable of secreting an anti-sheeppox virus K3L protein C-terminal monoclonal antibody, the anti-sheeppox virus K3L holoprotein monoclonal antibody secreted by the hybridoma cell line as well as application of the hybridoma cell line and the antibody. The preservation number of a hybridoma cell line K3L35 capable of secreting the anti-sheeppox virus K3L protein C-terminal monoclonal antibody in China Center for Type Culture Collection (CCTCC) is CCTCC NO: C2015223. The K3L protein C-terminal monoclonal antibody can be secreted by the hybridoma cell line K3L35. The hybridoma cell line K3L35 and the monoclonal antibody secreted by the hybridoma cell line K3L35 can be applied in preparation of a reagent for diagnosing or detecting sheeppox virus infection or a reagent for experiments.

Description

technical field [0001] The present invention relates to a monoclonal antibody and its application, in particular to a hybridoma cell strain capable of secreting a monoclonal antibody against the C-terminus of sheeppox virus K3L protein, and the C-terminal monoclonal antibody against sheeppox virus K3L protein secreted by it. Antibody, and the application of the hybridoma cell line and antibody. Background technique [0002] Goat pox is a highly contagious and devastating viral disease among sheep epidemics. It is listed as a legally notifiable animal disease by the World Organization for Animal Health (OIE). Difficulty and death are the hallmarks. Goat pox is mainly prevalent in some areas of Africa, the Middle East, India, Nepal and Turkey, and it is mainly prevalent in Qinghai, Gansu, Hunan and Inner Mongolia in China. Sheeppox virus (SPV) belongs to Poxviridae, Chordatepoxvirinae, and Goatpoxvirus genus. It is mainly transmitted through aerosol, close contact or insect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569C12R1/91
CPCC07K16/081C12N5/163
Inventor 赵志荀张强朱学亮张志东吴国华颜新敏李健朱海霞吴娜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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