Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Monoclonal antibody against sheep pox virus k3l protein and its application

A monoclonal antibody, sheep pox virus technology, applied to hybridoma cells or antibodies in the preparation of detection or detection of sheep pox virus reagents, the field of monoclonal antibodies against the whole protein of sheep pox virus K3L, can solve the economic problem of sheep farming industry loss, etc.

Active Publication Date: 2020-04-10
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sheeppox virus (SPV) belongs to Poxviridae, Chordatepoxvirinae, and Goatpoxvirus genus. It is mainly transmitted through aerosol, close contact or insect bites. Sheep are the main host of sheep poxvirus. All segments can be infected, but the death cases mainly occurred in lambs, and the mortality rate was as high as 100%, which caused significant economic losses to the sheep industry, and was considered to be an economically potential biological weapon.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Monoclonal antibody against sheep pox virus k3l protein and its application
  • Monoclonal antibody against sheep pox virus k3l protein and its application
  • Monoclonal antibody against sheep pox virus k3l protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention is explained below in conjunction with embodiments. The following content is only used to illustrate the relevant content details of the present invention, and should not be construed as limiting the protection scope of the present invention.

[0031] 1. Virus strains and cells

[0032] The strain used in the present invention is the Gulang strain of sheep pox virus, and the strain and Vero cells and SP2 / 0 cells used are all preserved by the applicant (Lanzhou Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences).

[0033] 2. Main reagents

[0034] pUC57 cloning vector, Top10 competent cells, plasmid extraction kit, XbaⅠ and BamHI restriction enzymes, T4 DNA ligase were purchased from Dalian Bao Bioengineering Co., Ltd.; DMEM medium, RPMI -1640 cell culture medium and fetal cattle Serum was purchased from Gibco; gel recovery kit was purchased from AXYGEN; pET11d expression vector, BL21 (DE3) plysS host bacteria, His binding resin and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a hybridoma cell line K3L25. The preservation number of the hybridoma cell line K3L25 in China Center for Type Culture Collection (CCTCC) is CCTCC NO: C2015222. The hybridoma cell line K3L25 and the monoclonal antibody secreted by the hybridoma cell line K3L25 show good immunogenicity, and can be applied in preparation of an early diagnosis reagent or a reagent for early diagnosing sheeppox virus infection or a reagent for basic tests.

Description

Technical field [0001] The present invention relates to a monoclonal antibody. Specifically, the present invention relates to a hybridoma cell strain against the sheeppox virus K3L protein and the monoclonal antibody secreted by the monoclonal antibody against the sheeppox virus K3L whole protein, and the hybridoma cells or Application of antibodies in preparation of reagents for detection or detection of sheeppox virus. Background technique [0002] Sheep pox is a highly contagious and devastating viral disease in sheep disease. It is listed as a legally reported animal disease by the World Organization for Animal Health (OIE). It is characterized by fever, conjunctivitis, rhinitis, skin and mucous membrane acne, respiratory Difficulties and death are characteristic. Sheep pox is mainly prevalent in some areas of Africa, the Middle East, India, Nepal and Turkey, and domestically, it is mainly prevalent in Qinghai, Gansu, Hunan and Inner Mongolia. Sheeppox virus (SPV) belongs t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569C12R1/91
CPCC07K16/081C12N5/163
Inventor 赵志荀朱学亮张强张志东吴国华颜新敏李健朱海霞吴娜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com