Method for in-vitro construction of tissue engineered nerves

Abstract

The invention provides a method for in-vitro construction of tissue engineered nerves. The method adopting a three-dimensional gravity bioreactor comprises the following steps of: culturing seed cells with nerve conduits under a three-dimensional micro-gravity environment, wherein the culture conditions are as follows: the seed cell suspension and nerve conduits are placed to a rotary culture container which is filled with complete medium, the cell final density is 1*10<6> / ml, the culture is carried out in a CO2 incubator of 37 DEG C, the rotation speed with the first 24 hours is 10rpm, so that the cells sufficiently contact and are adhered with the nerve conduits; adjusting the rotation speed of the three-dimensional micro-gravity bioreactor after 24 hours, so that the nerve conduits are suspended in the culture solution; and stopping the culture after 7-14 days to obtain the tissue engineered nerves. The method disclosed by the invention can be used for ensuring that the seed cells adhered with the nerve conduits are more and more uniformly, so that peripheral nerve regeneration is facilitated; and moreover, schwann cells, which are induced by skin-orientated precursor cells, are closer to the true schwann cells and are more suitable for being used as seed cells of nerve tissue engineering.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and specifically relates to a method for constructing tissue-engineered nerves in vitro, in particular to a method for constructing tissue-engineered nerves in vitro by using a three-dimensional microgravity bioreactor. Background technique [0002] Every year in the world, there are many cases of peripheral nerve extrusion, stretching, and contusion injuries due to trauma such as car accidents, sharp objects, and bullets. For short-distance peripheral nerve defects, tension-free surgery can be performed using the elasticity and curvature of the nerve itself. The broken end was sutured to repair the defect. When the defect exceeds a certain distance and cannot be sutured without tension, nerve grafting must be performed. At present, autologous nerve transplantation is the most commonly used clinically, and it is regarded as the gold standard of peripheral nerve repair. However, the sources...

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Problems solved by technology

[0005] At present, the mechanism of microgravity has not yet been elucidated, and different types of cells may respond quite differently to microgravity, and there is no literature on the attachment of different types of cells to complex structures under microgravity, so different types of cells have been developed. And the study of microgravity three-dimensional culture of complex structural scaffolds to prepare tissue engineered nerves, which has guiding significance for the repair of clinical nerve defects

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