Preparation method of oxidized grapheme/silver particle nanometer compound and application thereof

A nanocomposite and graphene technology, applied in botany equipment and methods, applications, animal repellants, etc., can solve the problems of high toxicity, endangering human health and ecological environment, etc., and achieve low toxicity, rapid preparation, water-soluble good sex effect

Inactive Publication Date: 2013-08-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In view of the fact that most of the reducing agents used in the existing preparation methods are chemical organic reagents, which are highly toxic and enda...

Method used

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  • Preparation method of oxidized grapheme/silver particle nanometer compound and application thereof
  • Preparation method of oxidized grapheme/silver particle nanometer compound and application thereof
  • Preparation method of oxidized grapheme/silver particle nanometer compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of antibacterial material graphene oxide / silver particle nanocomposite (GO-Ag).

[0031] Use 1g of expanded graphite as the initial raw material, add about 30 times the weight of sodium chloride solid and grind until there are no obvious visible particles, add water and suction filter, wash away the sodium chloride, and dry the finely ground graphite for later use. Add about 30mL of concentrated sulfuric acid (98.3%) into the flask, put in dried fine graphite, stir at room temperature for 8 hours, then place the mixture in an ice bath, add about 3g of potassium permanganate, and slowly raise the temperature to 115 ℃, slowly add about 150mL of water, then add about 10mL of 30% hydrogen peroxide and stir, centrifuge to remove the upper aqueous solution, and wash repeatedly until the solution no longer precipitates. Sonicate 5 mL of graphene oxide suspension with a concentration of 3 mg / mL for 30 min to obtain a clear graphene oxide hydrosol, then add NaOH soli...

Embodiment 2

[0034] The effect of the graphene oxide / silver particle nanocomposite (GO-Ag) obtained in Example 1 on the growth activity of bacteria was studied.

[0035] The graphene oxide / silver particle nanocomposite obtained in Example 1 was mixed with the bacterial suspension for culture, and after 2.5 hours, the bacterial activity was determined by the MTT method. The specific method is: Escherichia coli and Staphylococcus aureus were cultured in LB liquid medium respectively, and cultured in a constant temperature shaking window until the exponential growth phase (OD 600 When it is about 0.5), take 200μl to re-inoculate in 2ml of new medium, add different volumes of GO-Ag nanocomposites, so that the final concentrations of the three different ratios of the complexes are 2.5, 5, 10μg / ml, and put it into the constant temperature shaker Continue culturing in the bed for 2.5 hours, take the suspension and add 100 μl per well to a 96-well plate, then add 20 μl MTT, place the 96-well plate...

Embodiment 3

[0038] The effect of the graphene oxide / silver particle nanocomposite (GO-Ag) obtained in Example 1 on the number of bacteria was studied.

[0039] The graphene oxide / silver particle nanocomposite obtained in Example 1 was mixed with the bacterial suspension, and evenly spread on a solid LB agar plate after 2.5 hours, and the antibacterial activity of the material was analyzed by counting the number of colonies (CFU) obtained. The specific method is: Escherichia coli and Staphylococcus aureus were cultured in LB liquid medium respectively, and cultured in a constant temperature shaking window until the exponential growth phase (OD 600 0.5), take 200 μl to re-inoculate in 2ml of new medium, add GO-Ag nanocomposites with a ratio of 1, so that the final concentrations are 2.5, 5, 10 μg / ml respectively, put them in a constant temperature shaker and continue to cultivate h, the gradient concentration was diluted and evenly spread on solid LB agar plates, placed in a constant temper...

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Abstract

The invention provides a preparation method of oxidized grapheme/silver particle nanometer compound and application thereof, and an oxidized grapheme solution is prepared by an improved Hummers method, and silver nitrate solid powder is added and boiled, and trisodium citrate is added as reducing agent and stabilizing agent, and the solution is boiled for 1 hour continuously and washed out for 3 times by a ultrafiltration pipe. Compared with the prior art, the preparation method provided by the invention is simple and fast, and the prepared nanometer compound has the advantages of stable structure, good water-solubility and good antibiosis activity, and the product can be used as antibiosis material with high efficiency and safety.

Description

technical field [0001] The invention relates to a pharmaceutical preparation, in particular to a preparation method and application of a graphene oxide / silver particle nanocomposite. Background technique [0002] There are many kinds of microorganisms, including prokaryotic microorganisms (such as bacteria), eukaryotic microorganisms (such as fungi, algae and protozoa) and cell-free organisms (such as viruses). They are small in size but closely related to human life, production and survival. Involving health, food, medicine, industry and agriculture, environmental protection and many other fields. Among them, the infection of pathogenic microorganisms, especially bacteria, has brought great harm to the breeding industry and human health, and caused huge economic losses. Therefore, the research and practical application of antibacterial aspects have always been widely concerned. Antimicrobial agents in the traditional sense include antibiotics and chemical disinfectants (s...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01P1/00
Inventor 刘庄彭睿唐佳陈倩张帅许利耕
Owner SUZHOU UNIV
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