Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for extracting echinocandin compounds from fermentation broth

A technology of echinocandins and compounds, applied in the field of biopharmaceuticals, can solve the problems of many extraction steps, low final yield, unsuitable for large-scale production and use, achieve less solvent, reduce the use of solvent, and improve the extraction process The effect of short routes

Active Publication Date: 2015-12-16
ZHEJIANG CHANGHAI PHARM CO LTD
View PDF16 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem of this process is that there are many extraction steps and the final yield is relatively low; dangerous chemical solvents such as chloroform are used in the extraction process, which is not suitable for large-scale production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of lung candidin B0 (PneumocandinB0) fermented liquid

[0038] Strain: Zalerionarboricola (HCCB30111, CGMCCNo.5412) (see Chinese Patent Application No. 201210041247.9);

[0039] Incline medium: PDA;

[0040] Incline culture conditions: 28°C, 5 days;

[0041] Seed Medium (%): Glucose 2.5, KH 2 PO 4 0.9, yeast powder 0.5, pharmaceutical vehicle 1.0, 85% lactic acid 0.2ml, trace elements 0.1ml, pH6.0;

[0042] Seed culture conditions: Digging and inoculation, 25°C, 220rpm, 3 days;

[0043] Fermentation medium (%): sucrose 12.5, sodium glutamate 0.8, yeast powder 0.8, proline 1.5, KH 2 PO 4 0.15, magnesium sulfate heptahydrate 0.04, trace elements 1ml, MES buffer salt 1.5, pH5.3;

[0044] Fermentation conditions: 10% seed transfer, 25°C, 220rpm, 7 days of fermentation;

[0045] Trace element composition (g / L): ferrous sulfate heptahydrate 1.0, manganese sulfate monohydrate 0.2, calcium chloride dihydrate 0.1, boric acid 0.056, copper ch...

Embodiment 2

[0047] Embodiment 2: Preparation of WF11899A (FR901379) fermentation broth

[0048] Strain: Coleophoma empetri WF11899A (FR901379) (see "improvement of FR901379 production by mutant selection and medium optimization by Munekazu Kanda published in Vol. 107 No. 5, 530-534, 2009, Journal of Bioscience and Bioengineering")

[0049] Incline medium: PDA;

[0050] Incline culture conditions: 28°C, 7 days;

[0051] Seed medium (%): sucrose 1.0, drug vehicle 2.0, yeast powder 1.0, peptone 1.0, KH 2 PO 4 0.2, CaCO 3 0.2, Tween800.1, pH6.5;

[0052] Seed culture conditions: Digging and inoculation, 25°C, 220rpm, fermentation for 5 days;

[0053] Fermentation medium (%): starch 3.0, glucose 1.0, wheat germ 0.2, medicine medium 2.0, corn gluten powder 0.5, KH 2 PO 4 2.0, NaHPO 4 1.5, ZnSO 4 .7H 2 O0.001, pH6.7;

[0054] Fermentation conditions: 10% seed conversion, 25°C, 220rpm, 7 days.

[0055] Product confirmation of WF11899A (FR901379): After HPLC detection, the content of W...

Embodiment 3

[0056] Embodiment 3: the preparation of echinocandin B (EchinocandinB) fermented liquid

[0057] Strain: Aspergillus rugulovalvus (HCCB30110, CGMCCNo.5413) (see Chinese Patent Application No. 201210041876.1);

[0058] Incline medium: PDA;

[0059] Incline culture conditions: 28°C, 5 days;

[0060] Seed medium (%): glucose 1.0, glycerin 1.0, cottonseed powder 2.5, pH 6.3;

[0061] Seed culture conditions: Digging and inoculation, 25°C, 220rpm, fermentation for 8 days;

[0062] Fermentation medium (%): maltose 6.0, dextrin 2.0, cottonseed powder 3.0, yeast powder 1.5, soybean oil 0.2, glutamic acid 0.3, KH 2 PO 4 0.1, pH7.0;

[0063] Fermentation conditions: 10% seed conversion, 25°C, 220rpm, 7 days.

[0064] Product confirmation of Echinocandin B (Echinocandin B): After HPLC detection, the content of Echinocandin B (Echinocandin B) in the fermentation broth was 0.87g / l.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting an echinocandins compound from fermentation liquid. The method comprises the following steps of: (a) performing cross flow filtration on the fermentation liquor by using microfiltration membrane equipment, cleaning with water and then adding an extractant for extraction in the filtration process, and filtering to obtain filtrate containing the target object; (b) adsorbing the filtrate containing the target object by using macroporous resin and then prewashing with a second solvent and resolving to obtain resolving liquor containing the target object; (c) concentrating the resolving liquid containing the target object and removing a second solvent; (d) adding a third solvent to separate out the target object to obtain solid-liquid mixture containing the target object; and (e) filtering the solid-liquid mixture from which the target object is separated out and drying to obtain the target object powder. The method has the advantages that the process flow is short, the used solvent system is simple, the extractant can be returned into the microfiltration membrane for continuous extraction through a simple nanofiltration concentration step so as to realize indiscriminate cycle use of the extractant, the using amount of the solvent is greatly reduced and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to an industrial production method for extracting echinocandin compounds from fermentation broth. Background technique [0002] Fungi are conditional pathogens for healthy humans and are harmless to the human body under normal circumstances; however, when the body's resistance decreases, it may cause local or systemic fungal infections. In recent years, due to the clinical abuse of cytotoxic drugs, immunosuppressants, broad-spectrum antibiotics and hormonal drugs, not only the immunity of the patient's body has been suppressed, but also the micro-ecological balance in the body has been destroyed, making the originally non-pathogenic fungi dominant bacteria, thereby inducing fungal infection; coupled with the development of open treatment methods such as organ transplantation, interventional therapy, and intubation techniques in modern medicine, the incidence of fungal i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/56C07K1/34
Inventor 张磊杨洋郭冬初王小勇孙新强
Owner ZHEJIANG CHANGHAI PHARM CO LTD