Method for quantification of cholesterol in small dense low-density lipoprotein

A cholesterol and lipoprotein technology, applied in the field of quantification of cholesterol in small and dense low-density lipoprotein, can solve problems such as time required

Inactive Publication Date: 2013-09-18
KYOWA MEDEX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is easier to operate than the ultracentrifugation method, but it takes time to measure because fractional sedimentation is required

Method used

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  • Method for quantification of cholesterol in small dense low-density lipoprotein
  • Method for quantification of cholesterol in small dense low-density lipoprotein
  • Method for quantification of cholesterol in small dense low-density lipoprotein

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0091] Buffer, such as MOPS (pH7.0)

[0092] Cholesterol esterases such as LPL3

[0093] Cholesterol oxidase, such as CHO-PEL

[0094] Reagents for the determination of hydrogen peroxide, such as peroxidase, N-ethyl-N-(3-methylphenyl)-N'-succinylethylenediamide (EMSE) and 4-aminoantipyrine (4- AA)

[0095] Surfactants to study

example 2

[0097] Buffer, such as MOPS (pH7.0)

[0098] Cholesterol esterases such as LPL3

[0099] Cholesterol dehydrogenases such as CHDH5

[0100] Oxidized coenzymes such as NAD

[0101] [The reduced coenzyme assay reagent selected according to the needs, such as 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H - tetrazole monosodium salt (WST-3)]

[0102] Surfactants to study

example 3

[0104] Buffer, such as MOPS (pH7.0)

[0105] Cholesterol esterases such as LPL3

[0106] Cholesterol dehydrogenases such as CHDH5

[0107] Oxidized coenzymes such as NAD

[0108] Reduced coenzyme oxidases such as NADH oxidase

[0109] Hydrogen peroxide assay reagents such as peroxidase, EMSE and 4-AA

[0110] Surfactants to study

[0111] In addition, each of the above-mentioned reagents may contain an aqueous medium, a stabilizer, a preservative, an interfering substance eliminating agent, a reaction accelerator, and the like, which will be described later, if necessary.

[0112] 3) Judgment method of surfactant A

[0113] Add each lipoprotein fraction as a sample to the reaction cell (2 μL), then add the reagent (0.15 mL) described in the above 2) to start the reaction, heat at 37°C for 5 minutes, and measure the absorbance of the reaction solution to form a straight line Increased time, for example, the change in absorbance of the reaction solution after 5 minutes of ...

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Abstract

A method for determination of sdLDL-C, which comprises the steps of: (i) in a reaction solution containing a surfactant which preferentially inhibits the reaction of sdLDL-C with enzymes for cholesterol measurement such as cholesterol ester hydrolase, allowing the enzymes for cholesterol measurement to act on a sample to eliminate HDL-C, VLDL-C, CM-C and LgLDL-C in the sample; (ii) adding a reagent which causes the reaction of sdLDL-C remaining in the reaction solution after the above step (i) to form hydrogen peroxide or reduced coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme; and (iii) determining the sdLDL-C concentration in the sample from the measurement value in (ii) and a previously prepared calibration curve.

Description

[0001] The application number is 200780033470.6 (the international application number is PCT / JP2007 / 070172), the entry date of China's national phase is March 9, 2009 (the international application date is October 16, 2007), and the title of the invention is "small and Quantitative method for cholesterol in dense low-density lipoprotein" is a divisional application of the Chinese invention patent application. technical field [0002] The present invention relates to a method for quantifying cholesterol (hereinafter abbreviated as sdLDL-C) in small dense low-density lipoprotein (hereinafter abbreviated as sdLDL) in a sample and a kit for quantification. Background technique [0003] Lipoproteins present in the blood are broadly classified into high-density lipoprotein (abbreviated as HDL hereinafter), low-density lipoprotein (abbreviated as LDL hereinafter), very low-density lipoprotein (abbreviated as VLDL hereinafter) and chyle Microparticles (hereinafter abbreviated as CM)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/32C12Q1/26
CPCC12Q1/60G01N33/92C12Q1/26C12Q1/32
Inventor 荒武知子片山有基三岛慎刚
Owner KYOWA MEDEX CO LTD
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