Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for quantification of cholesterol in small dense low-density lipoprotein
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A cholesterol and lipoprotein technology, applied in the field of quantification of cholesterol in small and dense low-density lipoprotein, can solve problems such as time required
Inactive Publication Date: 2013-09-18
KYOWA MEDEX CO LTD
View PDF3 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
This method is easier to operate than the ultracentrifugation method, but it takes time to measure because fractional sedimentation is required
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0094] Reagents for the determination of hydrogenperoxide, such as peroxidase, N-ethyl-N-(3-methylphenyl)-N'-succinylethylenediamide (EMSE) and 4-aminoantipyrine (4- AA)
[0101] [The reduced coenzyme assayreagent selected according to the needs, such as 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H - tetrazole monosodium salt (WST-3)]
[0111] In addition, each of the above-mentioned reagents may contain an aqueous medium, a stabilizer, a preservative, an interfering substance eliminating agent, a reaction accelerator, and the like, which will be described later, if necessary.
[0112] 3) Judgment method of surfactant A
[0113] Add each lipoprotein fraction as a sample to the reaction cell (2 μL), then add the reagent (0.15 mL) described in the above 2) to start the reaction, heat at 37°C for 5 minutes, and measure the absorbance of the reaction solution to form a straight line Increased time, for example, the change in absorbance of the reaction solution after 5 minutes of ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
A method for determination of sdLDL-C, which comprises the steps of: (i) in a reaction solution containing a surfactant which preferentially inhibits the reaction of sdLDL-C with enzymes for cholesterol measurement such as cholesterolester hydrolase, allowing the enzymes for cholesterol measurement to act on a sample to eliminate HDL-C, VLDL-C, CM-C and LgLDL-C in the sample; (ii) adding a reagent which causes the reaction of sdLDL-C remaining in the reaction solution after the above step (i) to form hydrogenperoxide or reduced coenzyme, and measuring the formed hydrogenperoxide or reduced coenzyme; and (iii) determining the sdLDL-C concentration in the sample from the measurement value in (ii) and a previously prepared calibration curve.
Description
[0001] The application number is 200780033470.6 (the international application number is PCT / JP2007 / 070172), the entry date of China's national phase is March 9, 2009 (the international application date is October 16, 2007), and the title of the invention is "small and Quantitative method for cholesterol in dense low-density lipoprotein" is a divisional application of the Chinese invention patent application. technical field [0002] The present invention relates to a method for quantifying cholesterol (hereinafter abbreviated as sdLDL-C) in small dense low-density lipoprotein (hereinafter abbreviated as sdLDL) in a sample and a kit for quantification. Background technique [0003] Lipoproteins present in the blood are broadly classified into high-density lipoprotein (abbreviated as HDL hereinafter), low-density lipoprotein (abbreviated as LDL hereinafter), very low-density lipoprotein (abbreviated as VLDL hereinafter) and chyle Microparticles (hereinafter abbreviated as CM)...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
