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Method for establishing salicornia suspension cell line

A technology of suspension cells and Salicornia, applied in the direction of microorganism-based methods, plant cells, biochemical equipment and methods, etc., can solve the problems of establishing suspension cell lines without halophytes

Inactive Publication Date: 2013-09-25
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no report on the establishment of suspension cell lines of halophytes

Method used

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  • Method for establishing salicornia suspension cell line
  • Method for establishing salicornia suspension cell line
  • Method for establishing salicornia suspension cell line

Examples

Experimental program
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Effect test

Embodiment 1

[0068] Embodiment 1, acquisition and identification of salicornia suspension cell line

[0069] In this example, Salicornia sativa seeds were used as explants, and after callus induction culture, subculture and suspension culture, a Salicornia sativa suspension cell line was finally established. Among them, the inventors of the present invention optimized the state of the callus, the composition ratio of the hormones in the suspension medium, and the subculture inoculation amount during the suspension culture. details as follows:

[0070] 1. The effect of callus state on Salicornia salicornia suspension cell line

[0071] (1) Experimental method

[0072] 1. Disinfection of explants

[0073] Take salicornia seeds, peel off the outer shell, soak in solution A for 1 minute, then transfer to solution B for 15 minutes, then rinse with sterile water 7-8 times, complete disinfection, and set aside.

[0074] Solution A: a volume fraction of 75% ethanol in water;

[0075] Solution...

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Abstract

The invention discloses a method for establishing a salicornia suspension cell line. The method for establishing the salicornia suspension cell line comprises the following steps: (1) placing a salicornia explants onto an induction medium for induction culture so as to obtain callus 1; (2) placing the callus 1 onto a subculture medium to carry out subculture for more than five times so as to obtain callus 2; and (3) placing the callus 2 onto a suspension medium to carry out suspension and shake culture so as to obtain the salicornia suspension cell line. By the utilization of the method provided by the invention, the salicornia suspension cell line is established for the first time, thus being beneficial to deep analysis of salt tolerance mechanism of salicornia from the angle of cytology, laying a technical foundation for gene transformation and genetic modification of salicornia and providing a new possible way to gene function identification of salicornia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for establishing a salicornia suspension cell line. Background technique [0002] There have been many cultivations of plant suspension cells in the world, and the cultivation of suspension cells plays an important role in the improvement of science, medicine, agriculture and daily life; they are excellent cytology research materials, and can be used as bioreactors to synthesize related Secondary metabolites (flavoring agents, essences, etc.). At present, there have been many literature reports on the culture technology of suspension cells, among which there are not only various model plant suspension cell establishment schemes, but also suspension cell establishment schemes for various food crops and economic crops, which also involve some obstinate medicinal plants. Methods of cultivation of plants and woody plants. Cao Fuliang et al established Ginkgo biloba suspension ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12R1/91
Inventor 李银心陈显扬台方
Owner INST OF BOTANY CHINESE ACAD OF SCI
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