Method for rooting culture of sugarcane tissue culture seedling

A technology for rooting culture and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of limited application scope of tissue culture technology, complicated pollution control problems, unfavorable promotion and popularization, etc., to reduce the cost of rooting culture, simplify tissue culture links, The effect of low pollution rate

Inactive Publication Date: 2013-10-02
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the problem of pollution control is very complicated and expensive, which has become one of the important reasons restricting the industrialization of tissue culture

Method used

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  • Method for rooting culture of sugarcane tissue culture seedling
  • Method for rooting culture of sugarcane tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The method for rooting culture of sugarcane tissue culture seedlings of the present invention comprises the steps:

[0015] 1. Culture Vessel Preparation

[0016] Use the existing tissue culture container as the basic container, rinse and dry the water in the container naturally for later use;

[0017] 2. Medium Preparation

[0018] Take tap water to prepare MS + sodium hypochlorite 8000mg / L + white sugar 30g / L liquid medium, and then divide it into culture containers according to a certain amount, seal it, and set it aside.

[0019] 3. Rooting culture

[0020] Put the tissue-cultured seedlings into the above-mentioned culture medium and place them in a culture room for cultivation. After 20-30 days of cultivation, the tissue-cultured seedlings can grow into a complete root system.

[0021] 4. Implementation effect

[0022]

Embodiment 2

[0024] Another example of the method for sugarcane tissue culture seedling rooting culture of the present invention, comprises the steps:

[0025] 1. Culture Vessel Preparation

[0026] Use the existing tissue culture container as the basic container, rinse and dry the water in the container naturally for later use;

[0027] 2. Medium Preparation

[0028] Weigh 6-8 grams of agar per liter of medium and boil 200ml of tap water, prepare MS + sodium hypochlorite 1200mg / L + white sugar 30g / L medium, mix well with tap water, and pack in a certain amount in culture containers before the medium solidifies , seal and set aside.

[0029] 3. Rooting culture

[0030] Put the tissue-cultured seedlings into the above-mentioned culture medium and place them in a culture room for cultivation. After 20-30 days of cultivation, the tissue-cultured seedlings can grow into a complete root system.

[0031] 4. Implementation effect

[0032]

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PUM

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Abstract

The invention discloses a method for the rooting culture of a sugarcane tissue culture seedling. The method comprises the following steps of: inoculating a multiplication sterile tissue culture seedling in a culture medium of MS (Murashige-Skoog), sodium hypochlorite with the concentration being 800-1200mg/L and 30g/L of white sugar, sealing, placing the mixture in a culture room with the temperature of 27+/-1 DEG C, carrying out the rooting culture by illuminating for 12-16h every day, so as to realize the purpose that a complete root system grows after 20-30 days. According to the method disclosed by the invention, the culture medium does not contains exogenous hormones and does not need to be subjected to high-pressure sterilization, carries out the inoculation without an ultraclean working platform, and is simple and rapid to operate and low in pollution rate; the rooting culture cost can be reduced by 40-60 percent; the cultured rooting seedling grows strongly.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for rooting culture of sugarcane tissue culture seedlings. Background technique [0002] Plant tissue culture has: 1. Save a lot of space and time; 2. The cultivation process is not affected by external environmental factors, and can be carried out all year round; 3. Repeatability and other three advantages. It is often widely promoted and applied in the fields of large-scale propagation of virus-free seedlings, mutation breeding, transgenics, production of secondary metabolites, artificial seeds, etc., and has produced huge benefits. However, tissue culture technology has strict requirements on a sterile environment, resulting in a significant increase in application costs. The complex technical links and instruments in the tissue culture process, such as aseptic operating tables, biosafety cabinets or autoclaves, are all formulated around the prevention of contamin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李松刘俊仙淡明刘丽敏刘红坚杨柳余坤兴卢曼曼杨荣仲谭宏伟王维赞唐红琴陈庭速谭芳刘欢
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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