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Milk cow pregnancy colloidal gold detection test strip

A technology of colloidal gold test paper and detection test paper, which is applied in measurement devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2013-10-02
上海阿趣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] A simple and effective method for diagnosing early pregnancy in dairy cows has long been an urgent problem for cattle producers and animal husbandry and veterinary workers

Method used

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  • Milk cow pregnancy colloidal gold detection test strip

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Experimental materials

[0017] ① Dairy cow progesterone standard 1 (made by the company)

[0018] ② Dairy cow progesterone standard 2 (purchased from SIGMA)

[0019] ③ Progesterone monoclonal antibody: YT2013001, YT2013002 (made by the company)

[0020] ④ Goat anti-mouse polyclonal antibody (made by the company)

[0021] The above are the main experimental materials, including some conventional experimental reagents, instruments, etc.

[0022] experimental method

[0023] ① Preparation of colloidal gold solution:

[0024] First heat the 0.01% haucl4 solution to boiling, quickly add a certain amount of 1% trisodium citrate aqueous solution, some blue at first, then light blue, blue, then red after heating, and transparent orange-red after boiling for 7 to 10 minutes. The colloidal gold preparation of various particles is shown in Table 1.

[0025] haucl4 (ml) 1% trisodium citrate (ml) diameter (ml) 100 5.0 10.0 100 4.0 15.0 100...

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Abstract

The invention relates to a milk cow early pregnancy diagnosis detection test strip, and belongs to the field of disease immunodetection, wherein an early pregnancy diagnosis test can be simply and efficiently performed with the test strip. Experimental data show that the test strip has characteristics of high detection rate (more than 90%), low false positive rate (less than 1%), high sensitivity (0.375 mug / ml), high stability (quality is not significantly affected after storage for 24 months at a room temperature), and high practical application value, and can be used for milk cow early pregnancy diagnosis.

Description

technical field [0001] The invention is a colloid pregnancy test strip for dairy cow pregnancy, which is a simple and high-efficiency diagnostic test for early pregnancy. It can be applied to early pregnancy diagnosis of dairy cows. Background technique [0002] Colloidal gold immunochromatography assay (Gold immune-chromatography assay, GICA) is a solid-phase labeling immunoassay technology that organically combines colloidal gold labeling technology, immunoassay technology and chromatographic analysis technology. It uses colloidal gold as a marker and uses a special antigen-antibody reaction and a solid-phase membrane system for immunoassay. It consists of colloidal gold labeling technology and solid-phase membrane immunoassay system. [0003] Colloidal gold labeling technology is one of the four major immunolabeling techniques developed in the last century. It is widely used in electron microscopy, flow cytometry, molecular biology and even biochips. It is almost always...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
Inventor 不公告发明人
Owner 上海阿趣生物科技有限公司