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A protein related to rice blast resistance and its coding gene and application
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A protein and disease resistance technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of little research on pathogenic function
Inactive Publication Date: 2015-08-12
BEIJING UNIV OF AGRI +1
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Problems solved by technology
A lot of work has focused on the cloning of rice R genes and the ETI signaling pathway, but there is little research on non-specific resistance, especially the pathogenic function of PTI and pathogenic effector proteins
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Embodiment 1
[0066] Example 1. Acquisition of the cDNA sequence of the rice blast resistance-related gene OsBIK1
[0067]Take rice variety Nipponbare (Oryza sativa L.japonica.cv. Nipponbare, Sakai H, Lee SS, Tanaka T, et al. 2013. Rice Annotation Project Database (RAP-DB): An Integrative and Interactive Database for Rice Genomics. Plant Cell Physiol.54(2):e6. The public can obtain it from the Institute of Microbiology, Chinese Academy of Sciences.) as the experimental material, extract the total RNA from the tissues of roots, stems, leaves, spikes, and sheaths, mix them in equal amounts, and take 2 μg to reverse Transcribed into cDNA. Using this cDNA as a template and using bik1-F5'-ATGGGGAATTGCTGGGGCGC-3' and bik1-R5'-AACCAGCCTCGCATTTGCGG-3' as primers, the cDNA sequence of rice OsBIK1 protein was amplified by PCR.
[0068] Table 1 is the reaction system
[0069] wxya 2 o
18.3μL
10×PCR buffer
2.5μL
dNTP Mixture (2.5mmol / L)
2.5μL
Long Taq enzym...
Embodiment 2
[0081] Embodiment 2, the application of OsBIK1 gene
[0082] 1. Analysis of susceptible phenotypes of OsBIK1 gene mutants and controls
[0083] In order to study the function of the OsBIK1 gene in rice disease resistance positively, the OsBIK1 genome obtained in the above-mentioned Example 1 was searched for the rice mutant database (http: / / signal.salk.edu / cgi-bin / RiceGE), and obtained from South Korea Purchased rice OsBIK1 gene mutant (gene number: LOC_Os05g02020; order number: PFG_2A-60144.L;, address: Gynheung An, Department of Life Science, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea; Jeong Dong-Hoon, An Suyoung, Kang Hong-Gyu, Moon Sunok, Han Jong-Jin, Park Sunhee, Lee Hyun Sook, An Kyungsook, and An Gynheung. 2002. T-DNA Insertional Mutagenesis for Activation Tagging in Rice. Plant Physiology ,130,1–9), the loss-of-function mutant plants with T-DNA insertion after repeated screening ( image 3 A), the specific screening method is as ...
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Abstract
The invention discloses a gene related to resistance on rice blast as well as protein coded by the gene and an application of the gene. The gene related to resistance to rice blast, namely OsBIK1, is one of the following nucleotide sequences: 1) a sequence 1 in a sequence table; 2) a nucleotide sequence of a protein sequence shown in a sequence 2 in a coding sequence table; and 3) a nucleotide sequence which has more than 90% homology with the nucleotide sequence limited by the sequence 1 in the sequence table and is used for coding protein with a homogenous function. The protein coded by the gene related to resistance to rice blast contains an amino acid sequence shown in the sequence 2 in the sequence table or is formed by substituting, deleting or adding one or more amino acid residues on an amino acid residue sequence of the sequence 2, has the same activity of the amino acid sequence of the sequence 2 and is derived by the sequence 2. The gene coding protein related to resistance to rice blast, namely OsBIK1, has significance for breeding a disease-resistant plant variety.
Description
technical field [0001] The invention relates to a protein related to rice blast resistance, its coding gene and application. Background technique [0002] Rice is the most important food crop in my country. In agricultural production, due to the large-scale planting of a single variety and the evolution of the pathogenicity of pathogenic microorganisms, many varieties often quickly lose their resistance to mutated pathogens, resulting in disasters. Important diseases of rice, such as rice blast, sheath blight and rice blast, have become more and more important restrictive factors affecting the increase of rice yield. Breeders can only meet production demands by constantly updating disease-resistant varieties. However, due to limited sources of resistance and long conventional breeding cycles, disease resistance has increasingly become a bottleneck restricting the development of new varieties. [0003] Plant disease resistance molecular biology is a frontier subject that s...
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