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Method for simply researching root aphids

A technology of aphids and roots, applied in the field of research on agricultural pests, can solve the problems of destroying the living environment of research subjects, failure to complete data collection, research data error tests, etc., and achieve strong operability, comfortable and reliable parasitic environment, and easy observation Effect

Inactive Publication Date: 2015-03-11
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Root pests, whether they are big or small, mainly damage the roots of plants, hidden deep in the soil, protected by the soil, and not easy to observe
In traditional research, whether it is behavioral observation or data investigation, the root soil is usually counted by removing the root soil. The effect of this external force will destroy the normal living environment of the research subject, resulting in errors in the research data or failure of the experiment.
The resistance of root aphids to external force damage is particularly weak, and traditional methods can hardly complete data collection

Method used

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  • Method for simply researching root aphids
  • Method for simply researching root aphids
  • Method for simply researching root aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Cultivation method of Aphid spp. indica (potting method-simple cupping method)

[0031] The Indian aphid is a kind of aphid that harms the root system of apples. It is mainly distributed in Zhaotong City, Yunnan Province. In the cultivation of apple trees in Zhaotong City, seedlings of crabapple seedlings are used as rootstocks for grafting. After survival, the grafted seedlings are used for colonization. The root of the apple tree is actually the root system of the seedlings of crabapple, and the Indian aphid is parasitic on this part. Therefore the present invention selects crabapple seedlings as the host plant, and the crabapple seedlings of different seedling stages can be bought in Zhaoyang District, and different seedling ages can be selected according to research needs.

[0032] Plant the crabapple seedlings on a large-diameter flowerpot (14.5cm in diameter, 11.5cm in height) (the first container), and make a small round hole with a diameter of about 1...

Embodiment 2

[0036] In this implementation example, both the first container and the second container are treated as transparent ( image 3), and other conditions are the same as those in the first implementation example. By this implementation example, the statistics of the growth habit and physiological phenomenon data of aphids can also be carried out with a transparent container, and with respect to the implementation example, the transparent container can directly observe, without the need of each time as in the implementation example 1 All observations require the first container to be removed. Such experiments break with the common belief that root aphids can only be reared in dark conditions to be in line with their natural attributes. It is further illustrated by the following implementation examples that it is more convenient to use a transparent container for raising, and also obtain normal physiological data.

Embodiment 3

[0037] Embodiment 3: Determination of developmental duration and occurrence generation

[0038] The potted plants inoculated with aphids in implementation 2 were placed in an artificial climate box (Guangdong Medical Device Factory, RHL-250-GS, RH55-70%, light 12h), and the development history of Schizosporum indica was carried out. period of experiments, and calculated its developmental starting point temperature, effective cumulative temperature and annual generation generation. The experiment was set at 4 different temperatures, with 20-30 replicates (pots) per treatment, and 1 head per pot. The artificial inoculation method is adopted, that is, the 4th instar aphid or adult aphid is lightly inoculated on the roots of crabapple seedlings with a small wolf hair brush, and then re-soiled. Observe and record the state of each insect every day.

[0039] Some physiological indexes of aphids recorded at different temperatures were calculated according to the following methods, ...

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Abstract

The invention provides an experimental method for simply researching root aphids. The method is characterized by including the steps: (a), providing a device comprising a first container and a second container which are combined in a detachable manner, wherein, each of the first container and the second container is formed by enclosing of a bottom and a side wall and comprises an opening; (b), forming a small round hole 1cm in diameter in the bottom of the first container prepared in advance, and filling the second container with fine sand or fine soil in the thickness of 3-4cm; (c), planting a host plant, providing a crabapple seedling at the age of 2-3 months, planting the crabapple seedling in the first container, and penetrating root tips of a taproot system of the crabapple seedling out from the round hole in the bottom of the first container; and (d), inoculating the aphids on the root tips penetrating out of the round hole in the bottom of the first container, then placing the first container on the second container to enable the bottom of the first container to be located in the second container and seal the opening of the second container, forming a cavity between the bottom of the first container and the bottom of the second container, and allowing roots penetrating out of the round hole in the bottom of the first container to contact with the fine sand or fine soil in the second container.

Description

technical field [0001] The invention belongs to the field of agricultural plant protection, more specifically, to a device and method for studying agricultural pests. Background technique [0002] Root pests, whether they are big or small, mainly damage the roots of plants, which are hidden deep in the soil and protected by the soil, making it difficult to observe. In traditional research, whether it is behavioral observation or data investigation, the root soil is usually counted by removing the root soil. The effect of this external force will destroy the normal living environment of the research subject, resulting in errors in the research data or test failure. The resistance of root aphids to external force damage is particularly weak, and traditional methods can basically not complete data collection. The invention solves the above problems and provides researchers with a set of simple methods and devices for studying the aphids on the roots of crops. Contents of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01G13/00
Inventor 赵雪晴谌爱东李向永尹艳琼
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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