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Application of berberine in preparation of drugs inhibiting adipocyte autophagy

A technology of berberine and medicine is applied in the application field of berberine in the preparation of medicines for inhibiting autophagy of subcutaneous fat and visceral fat cells, and can solve the problems of no research report and the like

Inactive Publication Date: 2013-11-20
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0006] Whether berberine can inhibit autophagy in subcutaneous and visceral adipose tissue of mice fed a high-fat diet, there is no relevant research report in this regard

Method used

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  • Application of berberine in preparation of drugs inhibiting adipocyte autophagy
  • Application of berberine in preparation of drugs inhibiting adipocyte autophagy
  • Application of berberine in preparation of drugs inhibiting adipocyte autophagy

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Experimental program
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Embodiment Construction

[0017] Below in conjunction with embodiment, the present invention will be further described:

[0018] Berberine (Berberine) used in the experiment was purchased from Shanghai Drug Administration; C57BL / 6 mice were purchased from Shanghai Slack Experimental Animal Co., Ltd.

[0019] Thirty healthy male C57BL / 6 mice weighing about 15 g were divided into 3 groups: control group (CON, n=6), model group (HFD, n=12) and drug group (HFD+BBR, n= 12), the control group was given normal feed, and the model group and drug group were given high-fat feed. After 12 weeks of feeding, the drug group continued to use berberine (50mg / kg) by gavage on the basis of the previous diet, once a day. 4 consecutive weeks. The mice were killed, and the subcutaneous fat and perirenal fat were weighed, and the fat tissue was immediately stored in liquid nitrogen for detection in subsequent experiments. In the following figures: * represents P<0.05; ** represents P<0.01; *** represents P<0.001.

[0020...

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Abstract

Belonging to the field of medicine preparation, the invention in particular relates to application of berberine in preparation of drugs inhibiting subcutaneous adipose and visceral adipose cell autophagy. The invention takes high fat fed mice as experimental objects to test whether berberine inhibits autophagy of subcutaneous adipose and visceral adipose cells of the mice. Specifically, the process includes: feeding C57BL / 6 mice with high fat for 12 weeks, then dividing the mice into a high fat group and a berberine group, continuing the high fat diet and berberine gavage once a day for 4 weeks, using Western Blot to detect the change of autophagy related protein, and using Real-time PCR to detect the change of the corresponding mRNA level. Results show that: under high fat feeding, subcutaneous and visceral adipose tissue autophagy increases, and berberine could effectively inhibit subcutaneous adipose and visceral adipose autophagy of the high fat fed mice, thus providing the experimental data and theoretical basis for more reasonable application of berberine in treatment of obesity clinically.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and particularly relates to the application of berberine in the preparation of drugs for inhibiting autophagy of subcutaneous fat and visceral fat cells. Background technique [0002] Obesity refers to excessive accumulation and / or abnormal distribution of adipose tissue in the body, and weight gain. It is a chronic metabolic disease caused by the interaction of multiple factors including genetics and environment. It mainly refers to the increase in fat cell volume. Large, increased in number, or both increased in size and increased in number. The incidence of overweight and obesity has reached epidemic proportions in some developed countries and regions. According to statistics, about half of adults in Western countries are overweight and obese. The prevalence of obesity in my country is also rising rapidly. Obesity, as one of the main components of metabolic syndrome, is closely related t...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P3/04A61P3/10A61P9/00A61P9/12
Inventor 宁光张晓燕杨颖
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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