Preparation method of recombinant panda IL-6 immunological adjuvant

A technology of IL-6 and immune adjuvant, which is applied in the field of preparation of recombinant giant panda IL-6 immune adjuvant, can solve the problems of unsatisfactory vaccines, poor safety, and low immune efficiency
CN103409453AInactive Publication Date: 2013-11-27四川卧龙国家级自然保护区管理局 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
四川卧龙国家级自然保护区管理局
Publication Date
2013-11-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a recombinant panda IL-6 immunological adjuvant. The preparation method comprises the following steps of: cloning A1 panda IL-6 whole-genome; culturing A11 panda peripheral blood mononuclear cells in vitro; extracting an A12 total RNA (Ribonucleic Acid); synthesizing Al3cDNA; designing and synthesizing an A14 primer; carrying out PCR (Polymerase Chain Reaction) amplification on an A15 target gene; carrying out TA (trophoblast antigen) cloning on a PCR product of an A16 target gene; carrying out preliminary identification on A17 recombinant plasmids; and carrying out prokaryotic expression of A2 panda interleukin 6, and preparing a polyclonal antibody. The canine distemper is known as a destructive infectious disease and is the main fulminating infectious disease which threatens the population quantity and the life safety of pandas and seriously affects the healthy development of the pandas. The recombination panda IL-6 immunological adjuvant can be used for remarkably enhancing the immune effect of canine distemper vaccines and has important significance for the prevention and treatment of the canine distemper.
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Description

technical field

[0001] The present invention relates, in particular, to a preparation method of recombinant giant panda IL-6 (gpIL-6) immune adjuvant. Background technique

[0002] The giant panda is known as China's "national treasure" and is a world-class rare wild animal. However, it is on the verge of extinction. In addition to the destruction of the giant panda's habitat due to human factors, disease and death have become one of the key reasons for the current endangered giant panda population. Among the more than 40 kinds of diseases that giant pandas suffer from, canine distemper IL-6 is the most severe infectious disease that threatens the population and life safety of giant pandas. Vaccine immunization is currently the main measure to prevent and control CDV, but the vaccines currently used are not very ideal, and there are two problems of low immunization efficiency and poor safety. Therefore, improving the immunity of giant pandas under safe dose vaccination has ...

Claims

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