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Method for discriminating output performance of royal jelly by using honeybee he*71 gene fluorescent quantitative PCR technology

A fluorescence quantification and royal jelly technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of bee species and sample size, and sample selection is not very scientific, and achieve good yield performance

Inactive Publication Date: 2013-11-27
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

They believed that the number of bee species and sample size in the above experiment was too small, and the sample selection was not very scientific. It cannot be simply inferred that W316bp can be used as a marker of high-yielding royal jelly just because three bees with high-yielding royal jelly have it but not among the low-yielding bees. Potentially related to other biological traits of four different bee species

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  • Method for discriminating output performance of royal jelly by using honeybee he*71 gene fluorescent quantitative PCR technology

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Effect test

Embodiment 1

[0033] Example 1 A method utilizing honeybee hex71 gene fluorescent quantitative PCR technology to identify the yield performance of royal jelly, comprising the following steps:

[0034] , bee sample collection

[0035] ① During the jelly production season, 30 colonies of bees in Zhejiang Qiandao Lake Experimental Bee Farm were organized to produce royal jelly for 5 consecutive times, and the weight of royal jelly and the amount of royal jelly received by each colony were weighed and recorded.

[0036] ②5 colonies with high royal jelly production, 5 colonies with low royal jelly production, and 4 other bee colonies whose royal jelly production was between high and low yields with a gradient trend were screened out, and a total of 14 bee colonies were selected as the investigated bee colonies. The royal jelly production range of the royal jelly high-yield bee colony and the royal jelly low-yield bee colony is that the high-yield colony produces no less than 100 grams of roy...

Embodiment 2

[0080] Example 2, the expression abundance of hex71 gene in bees with high royal jelly production is significantly higher than that in bees with low production of royal jelly

[0081]By counting the royal jelly production of 30 colonies of bees in the apiary five times in a row, 5 colonies with high royal jelly production and 5 colonies with low royal jelly production were finally screened. The results showed that the average yield of royal jelly in five colonies and five times of low-yield royal jelly group was 64.32g, and the average yield of royal jelly in five groups of five times in high-yield royal jelly group reached 115.64g. The result shows F 1，40 =76.3935, P=0.0001, that is, there was a significant difference in the yield of royal jelly between the high and low yield groups of royal jelly.

[0082] The expression abundance of honeybee hex71 gene was detected by qRT-PCR technology. The expression abundance of the hex71 gene in the five colonies of bees in the high-y...

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Abstract

The invention relates to a method for discriminating output performance of royal jelly by using a honeybee he*71 gene fluorescent quantitative PCR technology. The method comprises steps of: acquisition of honeybee samples; extraction of head RNA of the honeybees; synthesis of the head cDNA of the honeybees; design of primers; qRT-PCR reaction system and condition; and discrimination of output performance of royal jelly. From the height of molecular biology, the invention provides a scientific and accurate fluorescent quantitative PCR method for discriminating output performance of royal jelly, aiming at the problem that production of royal jelly is critically influenced by conditions including bee colony potential, nectar source, environment and climate. In addition, the method can substantially shorten period for investigation of royal jelly production property, accelerate the speed of honeybee breeding, reduce cost of investigation of royal jelly production property and breeding cost, and mitigate working strength for field investigation of royal jelly output performance.

Description

technical field [0001] The invention relates to a method for identifying the yield performance of royal jelly by using fluorescent quantitative PCR technology, in particular to a method for identifying the yield performance of royal jelly by using the fluorescent quantitative PCR technology of honeybee storage protein (hex71). Background technique [0002] my country is a big beekeeping country, and it is also a major producer of royal jelly. More than 90% of the world's royal jelly is produced in China, and royal jelly-yielding bees are a unique bee resource in my country. For a long time, my country's bee industry scientific and technological workers have been committed to the related research on the genetic breeding of bees with high royal jelly yield, in order to find the genetic markers related to the high-yield traits of royal jelly, and serve the high-yield breeding of bee royal jelly. The genetic markers related to the high yield of royal jelly were studied at the le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 苏松坤潘娇刘小彦
Owner FUJIAN AGRI & FORESTRY UNIV
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