Method for improving yield of cordycepin in Verticillium Paecilomyces GZ

Abstract

The invention discloses a method for improving yield of cordycepin in Verticillium Paecilomyces, comprising: GZ is inoculated on a PDA inclined plane culture medium; at the temperature of 26 DEG C, GZ is cultured for 5d and activated for 3 times; then, the obtained GZ is inoculated into a PDA liquid seed culture medium in a v / v ratio of 5%; at 140 rpm and the temperature of 26 DEG C, the mixture is cultured in a shake flask for 3d to obtain the liquid female seed; the liquid female seed, in a v / v ratio of 5%, is inoculated in a fermentation culture medium containing 2% of white granulated sugar, 1% of maltose, 2% of beef extract and 3% of yeast extract; meanwhile, cetane, in a v / v ratio of 2-8%, is added; and at 140 rpm and the temperature of 26 DEG C, the cordycepin is obtained by culturing the mixture in the shake flask for 7d.The method only needs stable and simple fermentation condition, is easy for culturing thallus, has good effect on improving cordycepin output and can realize commercial scale production.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for increasing the content of cordycepin in Paecilomyces verticillium GZ by using a two-phase culture system. Background technique

[0002] Two-phase culture, also known as two-phase culture, two-phase fermentation, two-phase fermentation, cell culture-separation coupling process. By adding water-soluble or fat-soluble organic substances, or polymeric compounds with adsorption effect, to plant cells or microbial culture, the culture system forms two phases due to the difference in distribution coefficient, and the cells grow in one of the phases. Secondary metabolites are synthesized, and these products are released outside the cell through active or passive transport, and are adsorbed by another phase. Two-phase culture greatly reduces the inhibitory effect of the product itself on cell metabolism, increases the yield of metabolites, and protects the product from the infl...

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