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Method for improving yield of cordycepin in Verticillium Paecilomyces GZ

A technology of Paecilomyces verticillium and cordycepin, which is applied in the biological field to achieve the effects of good yield effect, reasonable technical route and wide adaptability

Inactive Publication Date: 2012-12-26
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Cordycepin is a class of nucleoside antibacterial substances isolated from Cordyceps militaris for the first time. It has a variety of biological activities, but cordycepin is a secondary metabolite with low yield, which limits its application.

Method used

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  • Method for improving yield of cordycepin in Verticillium Paecilomyces GZ

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Embodiment 1-3

[0024] A method for improving cordycepin content in Paecilomyces verticillium GZ, comprising the following steps:

[0025] (1) Preparation of spore suspension:

[0026] Paecilomyces verticillium GZ strain was inoculated on the slant medium, cultured at 26°C for 5 days, activated 3 times, washed the spores with sterile Tween-80 saline, transferred to a sterile Erlenmeyer flask with glass beads, oscillated, Fully break up the spores, remove mycelia by filtering with 4 layers of sterile lens paper, and make 10 spores 6 times the spore suspension.

[0027] (2) Preparation of liquid parent species:

[0028] Take the prepared spore suspension, connect it to the PDA liquid seed culture solution with 5% inoculation amount, 26°C, 140rpm, and shake the flask for 3 days to obtain the liquid mother seed.

[0029] (3) Fermentation:

[0030] GZ was connected on the PDA slant medium, cultured at 26°C for 5 days, activated 3 times, then inserted into the PDA liquid seed medium at 5% (v / v)...

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Abstract

The invention discloses a method for improving yield of cordycepin in Verticillium Paecilomyces, comprising: GZ is inoculated on a PDA inclined plane culture medium; at the temperature of 26 DEG C, GZ is cultured for 5d and activated for 3 times; then, the obtained GZ is inoculated into a PDA liquid seed culture medium in a v / v ratio of 5%; at 140 rpm and the temperature of 26 DEG C, the mixture is cultured in a shake flask for 3d to obtain the liquid female seed; the liquid female seed, in a v / v ratio of 5%, is inoculated in a fermentation culture medium containing 2% of white granulated sugar, 1% of maltose, 2% of beef extract and 3% of yeast extract; meanwhile, cetane, in a v / v ratio of 2-8%, is added; and at 140 rpm and the temperature of 26 DEG C, the cordycepin is obtained by culturing the mixture in the shake flask for 7d.The method only needs stable and simple fermentation condition, is easy for culturing thallus, has good effect on improving cordycepin output and can realize commercial scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the content of cordycepin in Paecilomyces verticillium GZ by using a two-phase culture system. Background technique [0002] Two-phase culture, also known as two-phase culture, two-phase fermentation, two-phase fermentation, cell culture-separation coupling process. By adding water-soluble or fat-soluble organic substances, or polymeric compounds with adsorption effect, to plant cells or microbial culture, the culture system forms two phases due to the difference in distribution coefficient, and the cells grow in one of the phases. Secondary metabolites are synthesized, and these products are released outside the cell through active or passive transport, and are adsorbed by another phase. Two-phase culture greatly reduces the inhibitory effect of the product itself on cell metabolism, increases the yield of metabolites, and protects the product from the infl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/40C12R1/79
Inventor 李祝任秀秀肖洋陈青周礼红刘吴娟
Owner GUIZHOU UNIV
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