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Preparation, purification method and application of fig crude polysaccharide

A fig crude polysaccharide and fig technology are applied in the fields of preparing α-glucosidase inhibitors, preparing fig crude polysaccharide, and purifying fig crude polysaccharide, and can solve the problems of slowing down production and absorption, reducing postprandial blood sugar peaks, etc.

Active Publication Date: 2016-05-04
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experimental basis for anti-tumor and other biological activities can be found in the literature, but no one has studied its inhibitory effect on glucosidase. An enzyme that hydrolyzes oligosaccharides such as sucrose, inhibits the activity of α-glucosidase, can slow down the production and absorption of glucose, and reduce the peak blood sugar after meals

Method used

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  • Preparation, purification method and application of fig crude polysaccharide
  • Preparation, purification method and application of fig crude polysaccharide
  • Preparation, purification method and application of fig crude polysaccharide

Examples

Experimental program
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Embodiment 1

[0076] Embodiment 1, a preparation method of crude polysaccharides from figs, the steps are as follows: take some dried figs and cut them into pieces, reflux with 95% ethanol to remove fat and pigment, and reflux the residue with 70% ethanol to remove small molecular impurities after suction filtration; then take The residue is used as the raw material for extracting fig polysaccharide; take the residue, extract it with water as a solvent under microwave conditions, filter it with suction, take the filtrate and concentrate it to 100Ml by rotary evaporation, add 95% ethanol and let it stand for 24h, and take the precipitate after centrifugation. After removing the protein by Sevage method, freeze-dry to obtain the crude polysaccharide of figs; when extracting, the solid-liquid ratio of residue and water is 1:20; the microwave power is 550W, the extraction time is 13min, and the amount of ethanol used for alcohol precipitation is 3.50% of the volume of the extract times.

Embodiment 2

[0077]Embodiment 2, a preparation method of crude polysaccharides from figs, the steps are as follows: take some dried figs and cut them into pieces, reflux with 95% ethanol to remove fat and pigment, and reflux the residue with 70% ethanol to remove small molecular impurities after suction filtration; then take The residue is used as the raw material for extracting fig polysaccharide; take the residue, extract it with water as a solvent under microwave conditions, filter it with suction, take the filtrate and concentrate it to 100Ml by rotary evaporation, add 95% ethanol and let it stand for 24h, and take the precipitate after centrifugation. After removing the protein by Sevage method, freeze-dry to obtain the crude polysaccharide of figs; when extracting, the solid-liquid ratio of residue and water is 1:23; the microwave power is 550W, the extraction time is 13min, and the amount of ethanol used for alcohol precipitation is 3.60% of the volume of the extract times.

Embodiment 3

[0078] Example 3, a method for preparing crude polysaccharides from figs, the steps are as follows: take some dried figs and cut them into pieces, reflux with 95% ethanol to remove fat and pigment, and reflux the residue with 70% ethanol to remove small molecular impurities after suction filtration; then take The residue is used as the raw material for extracting fig polysaccharide; take the residue, extract it with water as a solvent under microwave conditions, filter it with suction, take the filtrate and concentrate it to 100Ml by rotary evaporation, add 95% ethanol and let it stand for 24h, and take the precipitate after centrifugation. After removing protein by Sevage method, freeze-dry to obtain crude fig polysaccharide; during extraction, the solid-liquid ratio of residue and water is 1:22.45; the amount of ethanol used for alcohol precipitation is 3.55 times the volume of the extract.

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Abstract

The invention discloses a preparation method of fig crude polysaccharide, which comprises the following steps: taking dried fig, pulverizing, refluxing with 95% ethanol to remove grease and pigments, carrying out vacuum filtration, and refluxing the residues with 70% ethanol to remove small molecule impurities; taking the residues as the raw material for extracting fig polysaccharide; and taking the residues, extracting under microwave conditions by using water as a solvent, carrying out vacuum filtration, concentrating the filtrate to 100Ml by rotary evaporation, adding 95% ethanol, standing for 24 hours, centrifugating, taking the precipitate, removing proteins from the precipitate by a Sevage process, and carrying out freeze-drying to obtain fig crude polysaccharide. In the extraction process, the ratio of the residues to the water is 1:(20-23), the microwave power is 550W, and the extraction time is 13 minutes; and the ethanol for alcohol precipitation is 3.50-3.60 times by volume of the extracting solution. The invention also discloses a purification method and application of the fig crude polysaccharide.

Description

technical field [0001] The present invention relates to a kind of preparation method of polysaccharide, especially a kind of preparation method of fig crude polysaccharide; The present invention also relates to the purification method of the fig crude polysaccharide prepared by the aforementioned method; The present invention also relates to fig crude polysaccharide or pure fig polysaccharide in Use in the preparation of α-glucosidase inhibitors. Background technique [0002] Fig Ficuscarica.L belongs to the genus Moraceae, and the edible part is its dry receptacle, which not only tastes sweet, but also has a wide range of nutritional and medicinal values. According to reports, figs can be used to invigorate the stomach and clear the intestines, reduce swelling and detoxify, inhibit tumors, and improve the body's immunity. Figs are rich in polysaccharides, which have various physiological activities, such as anti-tumor, immune regulation, antibacterial, anti-oxidation, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10
Inventor 李盈蕾陈建华孙吉佑詹永成宋晓凯
Owner HUAIHAI INST OF TECH