Combination drug, preparation method and application of tripterygium annua
A technology of Tripterygium wilfordii and Artemisia annua, applied in the field of preparation of combined medicines of Tripterygium wilfordii, can solve the problems of easy accumulation of toxicity, irreversible lesions of reproductive organs, etc., and achieve the effect of low toxicity and good activity
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Embodiment 1
[0013] Example 1 Investigation on the supercritical extraction process of Artemisia annua Tripterygium wilfordii
[0014] Weigh 5 parts of 100.0g dried Artemisia annua and Tripterygium wilfordii (1:1) mixture, use methanol, ethanol, 75% ethanol, ethylene glycol and ethyl acetate as entrainers for extraction, and take one part without entrainment agent as a control;
[0015] Fill the above-mentioned mixture of Artemisia annua and Tripterygium wilfordii into a supercritical extraction kettle, heat and pressurize according to the program, and perform dynamic extraction. 2 The flow rate is 4.6L h -1 , the extraction temperature is 45°C, the extraction pressure is 25MPa, and the amount of entrainer is 2mL·g -1 , quantitatively pumped into the entrainer, and the extraction time was 100min; the product was collected from the analysis kettle, the organic solvent was recovered under reduced pressure, and the supercritical extract of the medicinal material Tripterygium annua was obtai...
Embodiment 2
[0020] The influence of the different extraction methods of embodiment 2 on the transfer rate of active ingredients
[0021] 1. Preparation of Artemisia annua Tripterygium officinalis to ethanol extract
[0022] The mixture of Artemisia annua and Tripterygium wilfordii (1:1) was extracted twice with 95% ethanol, and the amount of ethanol was 8 times that of the medicinal materials. The extraction time was 2 hours and 1.5 hours respectively. Boiling at 180°C and slightly boiling at 130°C, respectively 2, 1.5h. After extraction, pour out the dregs of the medicine and let it dry. The two extracts were combined. Concentrate the 95% ethanol extract under reduced pressure at a temperature of 50°C, a rotational speed of 34r / min, and a pressure of -0.095MPa, and concentrate to 2L. Take out 500mL of the reserved sample, evaporate it in a water bath until the temperature is 82.5°C, and then put it into an oven and dry it into a dry product (the temperature of the oven is 42.5°C) to o...
Embodiment 4
[0033] Example 4 Investigation of the Liver and Kidney Injury Effects on Mice
[0034] ICR mice, clean grade, half male and half female, body weight 18-22g, a total of 180 mice were randomly divided into 9 groups, namely: blank control group; Artemisia tripterygium medicine pair alcohol extract group; Artemisia tripterygium Herb pair supercritical extract Ⅰ; Artemisia tripterygium herb pair supercritical extract group II; Artemisia tripterygium herb pair supercritical extract group III; Artemisia tripterygium herb pair supercritical extract group IV; Rattan medicine supercritical extract group V, Tripterygium wilfordii supercritical extract group and Artemisia annua supercritical extract group. Each group of experimental animals was divided into two batches, 10 in each batch, half male and half male, one batch was administered for 5 days, and the other group was administered for 10 days. The dosage was clinically equivalent (18.00 g (crude drug) / kg), and the blank control gro...
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