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Glutaraldehyde degradation strain as well as preparation method and application thereof

A technology of glutaraldehyde and bacterial strains, which is applied in the field of environmental pollution control, can solve problems such as immature technical means, limited resources of glutaraldehyde-degrading bacteria, and impact on soil, and achieve rapid and complete degradation, which is convenient for expanding production and practical application. The effect of avoiding secondary pollution

Inactive Publication Date: 2015-06-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the problem of glutaraldehyde pollution has not received enough attention, and relevant technical measures, especially biodegradation methods and technical means, are still immature, especially the resources of glutaraldehyde-degrading bacteria are still relatively limited, which seriously affects the use of biodegradation technology to treat polluted soil. Effect

Method used

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  • Glutaraldehyde degradation strain as well as preparation method and application thereof
  • Glutaraldehyde degradation strain as well as preparation method and application thereof
  • Glutaraldehyde degradation strain as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0043] Example 1: Screening and identification of glutaraldehyde-degrading bacteria

[0044] 1) Isolation of glutaraldehyde-degrading bacteria:

[0045] 10g of the soil collected from the breeding pig farm of Huazhong Agricultural University was added to a triangular flask filled with 90ml of sterile water. After shaking the flask for 4 days, 100μl of the soil solution was applied to the solution containing 0.05% glutaraldehyde (v / v). Inorganic salt solid medium (KNO 3 1900mg / l, NH 4 NO 3 1650mg / l, KH 2 PO 4 170mg / l, MgSO 4 .7H 2 O 370mg / l, CaCl 2 .2H 2O 440mg / l); 28 ℃ constant temperature culture. The single colony growing on the plate was picked out for streak isolation and purification, and then re-applied to the above-mentioned inorganic salt plate containing glutaraldehyde for cultivation. After 3 times of separation and screening, the strains with stable and efficient degradation ability to glutaraldehyde were preserved and stored on the LB slant for future ...

Embodiment 2

[0065] Embodiment 2: Determination of efficient degrading bacteria to glutaraldehyde degradation ability

[0066] 1) Preparation of glutaraldehyde standard curve:

[0067] Use high performance liquid chromatography to detect the concentration of glutaraldehyde: first use 2,4-dinitrophenylhydrazine (DNPH) to derivatize glutaraldehyde into 2,4-dinitrophenylhydrazone, and then analyze 2,4-dinitrophenylhydrazone The 360nm ultraviolet absorption of nitrophenylhydrazone determines the content of DNPH, and further converts it into the content of glutaraldehyde. HPLC detection method: Chromatographic column: Diamonsil TM C 18 5m, 200mm*4.6mm; mobile phase: acetonitrile / water=70 / 30 (V / V); column temperature: 25°C; flow rate: 1.0mL / min; detection wavelength: 360nm (UV / DAD); injection volume: 20μl (Wang Miaofei, Rapid Analysis of Glutaraldehyde Residue in Chewing Gum by High Performance Liquid Chromatography, Modern Food Science and Technology, 2007, 23(12): 80-82).

[0068] Derivati...

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Abstract

The invention discloses a glutaraldehyde degradation strain as well as a preparation method and an application thereof. The preparation method of the glutaraldehyde degradation strain comprises the followings steps: A, collecting a soil sample from a pig farm, adding the soil sample to a triangular flask which is filled with sterile water, and shaking the flask for cultivation; B, after 3-5 days, getting and coating a soil solution on an organic salt solid culture medium which uses the glutaraldehyde as the only carbon source for constant-temperature cultivation; and C, picking out single colonies growing on an organic salt flat plate which uses the glutaraldehyde as the only carbon source, continuously carrying out scribing separation and purification, and then re-coating the solution on the organic salt flat plate containing the glutaraldehyde for carrying out cultivation. The strain LP12 which has stable and efficient degradation ability to the glutaraldehyde is obtained after separating and screening, and the strain LP12 is stored on an LB inclined plane for later use. An identification result shows that the LP12 is nitrogen pseudomonas. The glutaraldehyde in an environment can be quickly cleared by utilizing biodegradation action of the LP12, so that a blank in domestic glutaraldehyde contaminated soil governance is filled up. The strain is quick in reproduction speed, cheap in materials needed for expanding the cultivation, low in production cost, good in economic benefits, and wide in application prospect.

Description

technical field [0001] The invention belongs to the field of environmental pollution control. Specifically, it relates to a bacterial strain for efficiently repairing glutaraldehyde-contaminated soil, and also relates to a preparation method of a bacterial strain for efficiently repairing glutaraldehyde-contaminated soil, and also relates to a use of a glutaraldehyde-degrading bacterial strain in soil environment treatment. Background technique [0002] Glutaraldehyde is a five-carbon bisacetal compound, which is widely used in the fields of sterilization of surgical instruments, disinfection of environmental items, and sterilization and disinfection of food and tableware (Jiang Heju et al., 2007). Health hazards due to the inherent toxicity of glutaraldehyde itself or improper use have occurred. It is irritating to the skin, mucous membranes and respiratory tract, sensitizing in some populations, and can cause asthma and inflammatory or fibrotic lung disease. Various coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/38A62D101/28
Inventor 陈雯莉黄巧云骆萍
Owner HUAZHONG AGRI UNIV
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