Surface modified ferric oxide nucleus-gadolinium oxide shell composite nano particle, as well as preparation method and application of particle

A composite nanoparticle and iron oxide nanotechnology, which is applied to preparations for in vivo experiments, medical preparations with inactive ingredients, and inorganic inactive ingredients, etc., can solve problems such as insufficient detection, image distortion, and fast metabolism. To achieve the effect of enhanced magnetic resonance imaging, accurate diagnosis and high mechanical strength

Inactive Publication Date: 2013-12-25
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] 1. The clinically used contrast agents include: Feridex, which is an iron oxide magnetic nanoparticle, which is scanned by T2-weighted MRI mode with reduced signal contrast. When the local magnetism is large, the image will be distorted and distorted
The other is Magnevist, which is a complex of gadolinium. It is scanned by T1-weighted magnetic resonance imaging with enhanced signal contrast. The image is clear, but the metabolism is too fast to be fully detected.

Method used

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  • Surface modified ferric oxide nucleus-gadolinium oxide shell composite nano particle, as well as preparation method and application of particle
  • Surface modified ferric oxide nucleus-gadolinium oxide shell composite nano particle, as well as preparation method and application of particle
  • Surface modified ferric oxide nucleus-gadolinium oxide shell composite nano particle, as well as preparation method and application of particle

Examples

Experimental program
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Effect test

Embodiment l

[0042] Embodiment 1, preparation of a surface polyethylene glycol-modified iron oxide core-gadolinium oxide shell composite nanoparticle

[0043] According to Chem.Mater., 2003,15,1367; Angew.Chem., Int.Ed., 2005,44,123; or J.Mater.Chem., 2012,22,3311. Prepare γ-Fe 2 o 3 (or Fe 3 o 4 ) nano-solution.

[0044] Take 100 μL γ-Fe 2 o 3 (or Fe 3 o 4 ) nano solution (4wt%) was added to 20mL aqueous solution containing chitosan (CS) (number average molecular weight 500, deacetylation degree 90%) 10mg and EDTA 2mg, and slowly added a certain amount of ethanol dropwise to the system under stirring conditions , when the system changes from clear to colloidal solution, add 20 μL of glutaraldehyde (25%) to cross-link the amino groups on the surface of chitosan, and react overnight at room temperature to obtain cross-linked CS-EDTA-γ-Fe 2 o 3 (or Fe 3 o 4 ) composite nanospheres. The emulsion of the magnetic polymer microspheres was centrifuged for 30 minutes in an ultracentrif...

Embodiment 2

[0048] Example 2. Preparation of a surface polyethylene glycol-modified iron oxide core-gadolinium oxide shell composite nanoparticle

[0049] Take by weighing 0.25 g of chitosan with a number average molecular weight of 300,000 after refining and a degree of deacetylation of 90%, and dissolve it in 50 ml of an aqueous solution containing a calculated amount of acrylic acid. Among them: the ratio of the amino group in chitosan to the carboxyl group in acrylic acid is 1:1. After 15 minutes, install the condensing device and connect the condensed water. Take 200 μL of γ-Fe2O3 (or Fe3O4) nano solution (4wt%) and add it to the above mixed solution, stir vigorously for 10 minutes, quickly raise the temperature to 70oC, add a small amount of initiator: potassium persulfate, and keep it at 70oC for 2 hours to ensure the Acrylic acid reacts completely. Then add 0.5ml of glutaraldehyde (25wt%) to crosslink for 2h. The resulting emulsion is poured into a dialysis bag that can only pas...

Embodiment 3

[0053] Example 3, Preparation of iron oxide core-gadolinium oxide shell composite nanoparticles loaded with doxorubicin hydrochloride

[0054] Take 15 mg of lyophilized surface polyethylene glycol-modified iron oxide core-gadolinium oxide shell composite nanoparticle powder and 1.5 mg of doxorubicin hydrochloride, mix them in 20 mL of deionized water, stir at room temperature for 48 h, centrifuge and wash three times, and remove the upper liquid The surface polyethylene glycol-modified iron oxide core-gadolinium oxide shell composite nanoparticles loaded with doxorubicin hydrochloride are obtained. In the above drug-loaded composite nanoparticles, the drug-loaded amount is 11.2%, and the drug-loaded efficiency is 95.4%.

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Abstract

A ferric oxide nucleus-gadolinium oxide shell composite nano particle with the surface modified by polyethylene glycol is 50-300nm in average particle size, and uses ferric oxide as a nucleus and gadolinium oxide as a shell; the surface of a composite nano porous microsphere is modified by polyethylene glycol; the particle is hydrophilic, has better stability in a wide pH (Potential of Hydrogen) scope, and is biocompatible; the surface of the nano particle is regular; a plurality of ducts are formed in a shell wall; and the nano particle has a T1 and T2 dual-mode magnetic resonance imaging function. The ferric oxide nucleus-gadolinium oxide shell composite nano particle with the surface modified by polyethylene glycol has an obvious dual-mode imaging effect; a preparation method is simple, green and environment-friendly; raw materials are cheap; the particle can be inoculated with a target molecule to detect a tumor more accurately; and the particle has high application values and wide application prospects in the fields of materials, biology and medicine. The invention discloses the preparation method of the particle.

Description

technical field [0001] The invention relates to a multi-functional core-shell structure inorganic nano-contrast agent, specifically a kind of drug-carrying function, the core is iron oxide, the shell is gadolinium oxide, the hollow structure can load drugs, and it has T1 and T2 dual-mode magnetic properties. Inorganic nanoparticles for resonance imaging and their applications in biomedicine. Background technique [0002] In modern medical diagnosis, there are more and more means for disease inspection. The diagnosis of disease not only needs to clarify the location, scope and nature of the disease, that is, to make a localization, quantitative and qualitative morphological diagnosis of the disease, but also to reflect its For functional changes, it is even necessary to make a comprehensive and objective analysis and evaluation of the surrounding relationship of the lesion and the general condition. Therefore, modern medical imaging examination plays a vital role and has pen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K47/34A61K47/02
Inventor 丁寅梁晨李英韦玮陈洪渊
Owner NANJING UNIV
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