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Sphingomonas paucimobilis

A technology of single cell bacteria and less active sheaths, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems that the results cannot meet the actual needs of industrial production, and achieve the reduction of nitrate nitrogen and nitrite nitrogen, obvious degradation effect

Active Publication Date: 2015-02-04
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, aerobic denitrification has attracted more and more attention, and there are many screening studies on aerobic denitrification bacteria at home and abroad, but the results are still far from meeting the actual needs of industrial production.

Method used

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  • Sphingomonas paucimobilis
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Isolation screening and identification of embodiment 1 bacterial strain

[0010] 1. Culture medium:

[0011] Primary screening of BTB medium: beef extract 3.0g; peptone 10.0g; sucrose 15.0g; sodium chloride 20.0g; Teepol 1.0g; bromothymol blue 0.00006g; water 1000ml.

[0012] Nutrient broth medium: peptone 10g; beef powder 3g; sodium chloride 5.0g; glucose 1g; water 1000ml.

[0013] Screening medium: NaNO 2 0.1g (about 20mg / L NO 2 -N); Glucose 5.0g; K 2 HPO 4 1.0g; NaCl2.0g; MgSO 4 .7H 2 O0.5g; water 1000ml.

[0014] 2. Screening method

[0015] Dilute the sludge sample by the gradient dilution method, take 0.1mL and evenly spread it on the surface of the BTB medium, put it in a constant temperature incubator, and cultivate it at 30°C for 2-3 days, then pick it up with an inoculation loop to make the surrounding medium appear blue halo The single colonies in the circle were isolated and purified as the primary screening strains. A total of 13 single colonies we...

Embodiment 2

[0024] Example 2 Degradation experiment of sphingomonas paucimobilis NI1 to nitrite nitrogen

[0025] 1. Culture medium:

[0026] Activation medium: nutrient broth;

[0027] Screening medium: NaNO 2 0.1g (about 20mg / L NO 2 -N); Glucose 5.0g; K 2 HPO 4 1.0g; NaCl2.0g; MgSO 4 .7H 2 O0.5g; water 1000ml.

[0028] Detection medium:

[0029] High concentration nitrite nitrogen medium: NaNO 2 0.05g (about 10mg / L NO 2 -N); Glucose 5.0g; K 2 HPO 4 1.0g; NaCl2.0g; MgSO 4 .7H 2 O0.5g; water 1000ml.

[0030] Low concentration nitrite nitrogen medium: NaNO 2 0.025g (about 5mg / L NO 2 -N); Glucose 5.0g; K 2 HPO 4 1.0g; NaCl2.0g; MgSO 4 .7H 2 O0.5g; water 1000ml.

[0031] 2. Experimental method:

[0032] Pick a single colony of Sphingomonas paucimobilis NI1 on the plate and inoculate it into a test tube containing 8ml of nutrient broth, and place it in a shaker at 30°C and 205r / min for activation; transfer 5ml of the bacterial solution to 10ml without centrifuge at 4000...

Embodiment 3

[0036] Example 3 Sphingomonas paucimobilis NI1 treatment of aquaculture wastewater experiment

[0037] The NI1 strains screened by the present invention are activated and expanded, fermented in a nutrient broth medium in a liquid state, centrifuged to collect the bacteria sludge, added to a starch carrier, and dried to make 10 billion / g bacteria powder. The aquaculture wastewater was taken from loach pond aquaculture wastewater. Its nitrate nitrogen content was determined to be 45.62±1.15mg / L and nitrite nitrogen was 0.58±0.03mg / L. The experimental container was a plastic bucket, and the experimental water body was 10L. Add the bacteria powder of Sphingomonas paucimobilis NI1 at a mass ratio of 50ppm, and no bacteria powder is added to the control group. The experimental group and the control group are set up in three parallels, and the aeration is uninterrupted during the experiment, and the aeration volume of each container is adjusted to be consistent. , and the experimenta...

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Abstract

The invention relates to the technical field of microorganism application, and particularly provides a sphingomonas paucimobilis strain. The sphingomonas paucimobilis strain is preserved in China center for type culture collection (CCTCC) in Wuhan University, Wuhan, China on September 29, 2013 with the preservation number of CCTCC NO: M2013456. The sphingomonas paucimobilis NI1 strain has an aerobic denitrification capacity, has simple nutritional requirements, can remarkably reduce nitrate nitrogen and nitrite nitrogen in a water body, can also effectively lower the content of ammonia nitrogen in domestic sewage, can be developed into an aquatic product microecological preparation, and has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of Sphingomonas paucimobilis and its application in water body purification. Background technique [0002] In recent decades, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. However, due to the accumulation of a large amount of residual bait, feces, and animal and plant corpses in the aquaculture water body, the nitrogen content in the water body seriously exceeds the standard, and the eutrophication intensifies, which destroys the aquaculture ecological environment, frequently causes fish and shrimp diseases, and eventually causes huge economic losses. . Studies have shown that the main cause of aquatic animal disease is the serious excess of nitrite nitrogen content in aquaculture water. Nitrite can oxidize ferrous hemoglobin in fish and shrimp, ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 王鹏甄丽郑玉莲
Owner QINGDAO VLAND BIOTECH GRP
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