Microorganism-loaded carbon source slow-release wax as well as preparation method and application thereof
A microbial and carbon source technology, applied in the field of water treatment, can solve the problems of microbial metabolic imbalance, unfavorable growth and reproduction of beneficial microorganisms, and deterioration of water quality, so as to maintain the balance of carbon, nitrogen and phosphorus, facilitate growth and reproduction, and promote growth and reproduction.
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Embodiment 1
[0069] The bacterial strain provided by the present invention has been identified as belonging to the genus Paenibacillus (Paenibacillus), named DFB2-6, and has been deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on August 21, 2020, and its preservation number is CGMCC No. .20535.
[0070] The biological properties of bacterial strain DFB2-6 of the present invention are as follows:
[0071] (1) Genotype:
[0072] ①The 16S rDNA sequence is shown in SEQ ID NO.1, with a total length of 1441bp, compared on the EzBioCloud website, and the most similar strain is Paenibacillus harenae KCTC 3951 T , the similarity is 94.47%;
[0073] ② Genome G+C content is 51mol%.
[0074] (2) Phenotype:
[0075] ① Morphological characteristics: Gram-positive bacteria, cultured on LB solid medium at 30°C for 3 days, the colonies are beige, with irregular edges, raised surface, opaque, and 1.5-2.0 mm in diameter. The temperature growth range is 0-9...
Embodiment 2
[0084] A carbon source slow-release wax loaded with microorganisms, including a waxy carrier, a water-insoluble microbial carbon source and an auxiliary agent distributed in the waxy carrier, and Paenibacillus harenae DFB2-6 distributed in the waxy carrier .
[0085] The waxy carrier includes the following raw materials in parts by weight: 70 parts of soft wax with a melting point of 45°C, 20 parts of paraffin wax with a melting point of 55°C, and 10 parts of microcrystalline wax with a melting point of 60°C.
[0086] The water-insoluble microbial carbon source includes the following raw materials: engine oil and PHBV; the mass of the engine oil and PHBV are respectively 5% and 2% of the mass of the waxy carrier.
[0087] The auxiliary agent includes the following raw materials: rhamnolipid and water; the mass of the rhamnolipid and water are respectively 1.5% and 2% of the mass of the waxy carrier.
[0088] The content of Paenibacillus is 8×10 per gram of wax carrier. 5 cfu...
Embodiment 3
[0095] A carbon source slow-release wax loaded with microorganisms, including a waxy carrier, a water-insoluble microbial carbon source and additives distributed in the waxy carrier, and calcium alginate fibers distributed in the waxy carrier in a three-dimensional network structure net, and Paenibacillus harenae DFB2-6 distributed in the waxy carrier; the calcium alginate fibers in the calcium alginate fiber mesh extend to the surface of the waxy carrier to communicate with the outside world.
[0096] The waxy carrier includes the following raw materials in parts by weight: 70 parts of soft wax with a melting point of 45°C, 20 parts of paraffin wax with a melting point of 55°C, and 10 parts of microcrystalline wax with a melting point of 60°C.
[0097] The water-insoluble microbial carbon source includes the following raw materials: engine oil and PHBV; the mass of the engine oil and PHBV are respectively 5% and 2% of the mass of the waxy carrier.
[0098] The auxiliary agent...
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