Microorganism-loaded carbon source slow-release wax as well as preparation method and application thereof

A microbial and carbon source technology, applied in the field of water treatment, can solve the problems of microbial metabolic imbalance, unfavorable growth and reproduction of beneficial microorganisms, and deterioration of water quality, so as to maintain the balance of carbon, nitrogen and phosphorus, facilitate growth and reproduction, and promote growth and reproduction.

Pending Publication Date: 2021-03-26
HANGZHOU XIUCHUAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, direct dosing of carbon sources will lead to a short-term increase in COD in the water body, resulting in hypoxia in the water body and deterioration of water quality, and only when the ratio of carbon to nitrogen and phosphorus is in an appropriate range can beneficial microorganisms grow and reproduce rapidly. Directly adding carbon sources will cause the organic carbon content in the water body to be too large in the early stage and too small in the later stage, leading to problems such as microbial metabolism imbalance, which is not conducive to the growth and reproduction of beneficial microorganisms

Method used

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  • Microorganism-loaded carbon source slow-release wax as well as preparation method and application thereof
  • Microorganism-loaded carbon source slow-release wax as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The bacterial strain provided by the present invention has been identified as belonging to the genus Paenibacillus (Paenibacillus), named DFB2-6, and has been deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on August 21, 2020, and its preservation number is CGMCC No. .20535.

[0070] The biological properties of bacterial strain DFB2-6 of the present invention are as follows:

[0071] (1) Genotype:

[0072] ①The 16S rDNA sequence is shown in SEQ ID NO.1, with a total length of 1441bp, compared on the EzBioCloud website, and the most similar strain is Paenibacillus harenae KCTC 3951 T , the similarity is 94.47%;

[0073] ② Genome G+C content is 51mol%.

[0074] (2) Phenotype:

[0075] ① Morphological characteristics: Gram-positive bacteria, cultured on LB solid medium at 30°C for 3 days, the colonies are beige, with irregular edges, raised surface, opaque, and 1.5-2.0 mm in diameter. The temperature growth range is 0-9...

Embodiment 2

[0084] A carbon source slow-release wax loaded with microorganisms, including a waxy carrier, a water-insoluble microbial carbon source and an auxiliary agent distributed in the waxy carrier, and Paenibacillus harenae DFB2-6 distributed in the waxy carrier .

[0085] The waxy carrier includes the following raw materials in parts by weight: 70 parts of soft wax with a melting point of 45°C, 20 parts of paraffin wax with a melting point of 55°C, and 10 parts of microcrystalline wax with a melting point of 60°C.

[0086] The water-insoluble microbial carbon source includes the following raw materials: engine oil and PHBV; the mass of the engine oil and PHBV are respectively 5% and 2% of the mass of the waxy carrier.

[0087] The auxiliary agent includes the following raw materials: rhamnolipid and water; the mass of the rhamnolipid and water are respectively 1.5% and 2% of the mass of the waxy carrier.

[0088] The content of Paenibacillus is 8×10 per gram of wax carrier. 5 cfu...

Embodiment 3

[0095] A carbon source slow-release wax loaded with microorganisms, including a waxy carrier, a water-insoluble microbial carbon source and additives distributed in the waxy carrier, and calcium alginate fibers distributed in the waxy carrier in a three-dimensional network structure net, and Paenibacillus harenae DFB2-6 distributed in the waxy carrier; the calcium alginate fibers in the calcium alginate fiber mesh extend to the surface of the waxy carrier to communicate with the outside world.

[0096] The waxy carrier includes the following raw materials in parts by weight: 70 parts of soft wax with a melting point of 45°C, 20 parts of paraffin wax with a melting point of 55°C, and 10 parts of microcrystalline wax with a melting point of 60°C.

[0097] The water-insoluble microbial carbon source includes the following raw materials: engine oil and PHBV; the mass of the engine oil and PHBV are respectively 5% and 2% of the mass of the waxy carrier.

[0098] The auxiliary agent...

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Abstract

The invention relates to the technical field of water treatment, and discloses microorganism-loaded carbon source slow-release wax as well as a preparation method and an application thereof. The carbon source slow-release wax comprises a waxy carrier, a non-water-soluble microbial carbon source and an auxiliary agent which are distributed in the waxy carrier, and paenibacillus distributed in the waxy carrier, The paenibacillus is named as DFB2-6, is preserved in China General Microbiological Culture Collection Center on August 21, 2020, has a preservation number of CGMCC No.20535, and is namedas Paenibacillus harenae in a classified manner. The carbon source slow-release wax can slowly release a carbon source after being put into a to-be-treated water body, cannot cause increase of COD (Chemical Oxygen Demand) of the water body, can provide a continuous, on-demand and controllable carbon source for the paenibacillus, keeps balance of carbon, nitrogen and phosphorus, and is beneficialto growth and propagation of the paenibacillus.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a carbon source slow-release wax loaded with microorganisms, a preparation method and application thereof. Background technique [0002] Nitrogen is an important nutrient source for organisms, and excessive nitrogen content in water bodies can easily lead to eutrophication of water bodies. There are many sources of nitrogen pollution, and industrial and mining wastewater, livestock and poultry breeding wastewater, and domestic wastewater all have high nitrogen content. At present, nitrogen pollution has become an important issue in the prevention and control of water pollution. [0003] Ammonia oxidizing bacteria, nitrosifying bacteria, nitrifying bacteria, denitrifying bacteria and other microorganisms can convert ammonia nitrogen, nitrite nitrogen, nitrate and organic nitrogen in the water body into nitrogen gas and overflow from the water body, thereby reducing the ni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N1/20C02F3/34C12R1/01C02F101/38C02F101/16
CPCC02F3/34C02F2101/16C02F2101/163C02F2101/166C02F2101/38C02F2305/06C12N1/20C12N11/10
Inventor 张文武苏悦郑刚刘鹏程王伟杨勇
Owner HANGZHOU XIUCHUAN TECH CO LTD
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