A rapid detection method for plasticizers in food based on in-situ spraying method
A detection method and plasticizer technology, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of no plasticizer content standard, inability to distinguish, etc.
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specific Embodiment 1
[0035] A rapid detection method for whether plasticizers in food exceed the standard based on in-situ spraying method, the flow chart is as follows figure 1 shown. The method includes the following steps:
[0036] a. Establish critical plasticizer inspection standards
[0037] Select solute: plasticizer;
[0038] Equipped with solvent: weigh 1g of hydroxylammonium hydrochloride, 8mg of sodium metavanadate, 0.16g of nickel nitrate hexahydrate, 20ml of 50% acetic acid, stir and mix evenly;
[0039] The mass ratio of solute and solvent is mixed according to the critical standard of plasticizer content;
[0040] Spread the mixed solution evenly on the cover glass, bake it with a baking lamp at 100°C for 10 minutes, and irradiate it with an ultraviolet lamp with a wavelength of 340nm to obtain blue fluorescence. The gray value of the fluorescence is used as the inspection standard; A blue color guide of the same color as the fluorescent color is used as standard.
[0041] This...
specific Embodiment 2
[0048] The difference between this embodiment and the specific embodiment 1 is that the grayscale value of the fluorescence is used as the inspection standard; specifically, the grayscale or RGB value of the fluorescence is obtained as a standard by using a machine vision method.
[0049] This machine vision method is convenient for automatic and rapid judgment, and can effectively avoid detection errors caused by subjective judgments and improve detection reliability.
specific Embodiment 3
[0050] The difference between this embodiment and specific embodiment 1 is that after step a is executed, the critical plasticizer inspection standard has been established, therefore, step a does not need to be repeated for each inspection, therefore, the steps of the method are as follows:
[0051] Spread the same reaction solution as the solvent used in step a evenly on the cover glass, bake at 100°C for 10 minutes with a baking lamp, and irradiate with a UV lamp with a wavelength of 340nm, if:
[0052] If no blue fluorescence is obtained, there is no plasticizer;
[0053] Obtain blue fluorescence, but lighter than the color of step a, then the plasticizer content does not exceed the standard;
[0054] Obtain blue fluorescence, but darker than the color of step a, then the content of plasticizer exceeds the standard.
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