Method for separating and culturing animal bone marrow mesenchymal stem cell
A kind of stem cell, isolation and culture technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of high experimental conditions, limited wide application, high cost and technical difficulty, etc.
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Embodiment 1
[0040] Embodiment 1, the method for isolating and culturing animal bone marrow mesenchymal stem cells
[0041] Three methods of isolating and culturing animal bone marrow mesenchymal stem cells were used in this implementation, namely whole bone marrow adherent method (abbreviated as BM), bone fragment digestion method (abbreviated as Bone) and bone marrow plus bone fragment method (abbreviated as BM+Bone). The inoculum for all three methods was derived from the femur of the same C57BL / 6 mouse. The inoculum of the whole bone marrow adherence method is the bone marrow of the isolated mouse femur, and the inoculum of the bone chip digestion method is the bone chip of the mouse femur. The bone chip is prepared as follows: remove the bone marrow in the whole bone marrow The remaining femur (the remaining part after removing the bone marrow of the complete femur) was cut into 1 cubic millimeter particles in 0.01M, pH7.2-7.4 PBS, added 0.1% type II collagenase, and placed at 37°C, ...
Embodiment 2
[0067] Embodiment 2, the method for isolating and culturing animal bone marrow mesenchymal stem cells
[0068] Three methods for isolating and culturing animal bone marrow mesenchymal stem cells were used in this implementation, namely the whole bone marrow adherent method, the bone slice digestion method and the bone marrow plus bone slice method. The inoculum for all three methods was derived from the tibia of the same C57BL / 6 mouse. The experimental methods of these three methods are the same as in Example 1 except that the femur is replaced by the tibia. The experimental results of these three methods are not significantly different from those in Example 1.
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