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A detection method and application of molecular markers related to piglet diarrhea resistance

A technology for resistance-related, piglet diarrhea, applied in the field of molecular biology

Inactive Publication Date: 2016-04-20
陆丰超发种畜有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few related studies on the porcine GPI1 gene at home and abroad

Method used

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  • A detection method and application of molecular markers related to piglet diarrhea resistance
  • A detection method and application of molecular markers related to piglet diarrhea resistance
  • A detection method and application of molecular markers related to piglet diarrhea resistance

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Embodiment 1

[0022] 1. Primer Design

[0023] Based on the first exon of the pig GPI1 gene (GenBank accession number JX125039) using comparative genomics, the gene was used to perform BLAST in GenBank, and the candidate SNPs of the GPI1 gene were screened after sequence alignment, and the first exon of the GPI1 gene was selected. For the 625bp candidate SNPs site, use the JX125039.seq sequence as the standard, design primers, and use PCR-RFLP technology to verify the existence of the site.

[0024] Primer sequence M-F: 5'-CTTGGAGCTAAGCAGTGATGTG-3',

[0025] M-R: 5′-CTGAGAGGTGTGATGAGGTCTG-3′

[0026] 2. DNA extraction under PCR conditions

[0027] The DNA samples came from a total of 183 pigs of 4 breeds, including 26 Duroc pigs, 55 Large White pigs, and 34 Landrace pigs from Xinwufeng Original Breeding Pig Farm; 68 Ningxiang pigs from the local breed. A small piece of pig ear tissue was taken to extract DNA.

[0028] PCR conditions:

[0029] PCR reaction system (total volume 20μL): 10...

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Abstract

The invention belongs to the technical field of preparation of a molecular marker of livestock and particularly relates to detection and application of a molecular marker about resistance characters of piglet diarrhea as piglet marker assisted selection. The molecular marker is obtained by gene cloning, and the nucleotide sequence of the molecular marker is shown in a sequence table SEQ ID NO:1. A base of G / T at the 625bp of the sequence table SEQ ID NO:1 is mutated so as to cause polymorphism of PCR-RFLP-BshN1. The invention also discloses a primer for amplifying a DNA sequence of genes and a detection method for polymorphism. The new molecular marker is provided for marker assisted selection on the resistance characters of piglet diarrhea.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a nucleic acid molecule comprising piglet gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to a single nucleotide polymorphism site in the polynucleotide sequence shown in SEQ ID NO: 1 and a method for detecting the single nucleotide polymorphism site. Background technique [0002] Studies have shown that GPI is related to many diseases of organisms. Known to be associated with human deposition in senile dementia, infectious protein diseases (including human Creutzfeld-Jakob disease, Gerstmann-straussler-scheinker syndrome, Kuru disease and pruritus in sheep and goats, etc.), rheumatoid arthritis, African sleeping sickness, malaria, paroxysmal nocturnal hemoglobinuria, chronic gastritis, peptic ulcer and gastric cancer are directly related. [0003] In the study of drug resistance genes of Candida albicans, GPI1 was considered to be related t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/68C12N15/11C12N15/10
Inventor 何俊张珈榕马海明蒋隽贺长青
Owner 陆丰超发种畜有限公司