Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof

A mannanase, detergent technology, applied in detergent compositions, detergent compounding agents, biochemical equipment and methods, etc., can solve problems such as unsuitability for formulations and washing conditions

Inactive Publication Date: 2014-01-22
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, mannanases may have a limited pH and / or temperature range in which they are active, which may make them unsuitable for certain formulations and wash conditions

Method used

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  • Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof
  • Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof
  • Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof

Examples

Experimental program
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example

[0252] The following examples are provided to demonstrate and illustrate certain preferred embodiments and aspects of the invention and should not be construed as limiting.

[0253] In the following experimental disclosures, the following abbreviations are used: M (mole / liter), mM (millimol / liter), μM (micromoles / liter), nM (nanomoles / liter), mol (mole), mmol (mmol ), μmol (micromole), nmol (nanomole), g and gm (gram), mg (milligram), μg (microgram), pg (picogram), L (liter), ml and mL (milliliter), μl and μL (microliter), cm (centimeter), mm (millimeter), μm (micrometer), nm (nanometer), U (unit), MW (molecular weight), s (second), min (minute), h (hour ), ℃ (Celsius), QS (sufficient), ND (not performed), rpm (rev / min), H 2 O (water), dH 2 O (deionized water), HCl (hydrochloric acid), aa (amino acid), bp (base pair), kb (kilobase pair), kD (kilodalton), MgCl 2 (magnesium chloride), NaCl (sodium chloride), Ca (calcium), Mg (magnesium), HEPES (4-(2-hydroxyethyl)-1-piperazine...

example 1

[0255] Cloning of Glycosyl Hydrolase Gte Man1 from Geobacillus thermophilicis

[0256] Select Geobacillus thermophile ( et al., IJSEM, 54:2361-2368, 2004 ( et al., International Journal of Systematic and Evolutionary Microbiology, Vol. 54, pp. 2361-2368, 2004)) as a potential source of diverse glycosyl hydrolases and other enzymes for industrial applications. Genomic DNA for sequencing was obtained by incubating Geobacillus thermophilic strain DSM16325 on a heart infusion agar plate (Difco) at 50°C for 24 hours. Cellular material was scraped from the plates and used to prepare genomic DNA with the aid of the ZF Fungal / Bacterial DNA Miniprep Kit from Zymo (Cat# D6005). The genomic DNA was used for genome sequencing and used to amplify the Gte Man1 gene for expression cloning. use Whole genome sequencing of Geobacillus thermophilic strain DSM16325 (obtained from DSMZ, Germany) by sequencing-by-synthesis technology (www.baseclear.com / sequencing / illumina-sequencing / ). G...

example 2

[0323] Expression of Glycosyl Hydrolase Gte Man1 from Geobacillus thermophilicis

[0324] The Gte Man1 gene was amplified from genomic DNA of Geobacillus thermophilicis using the following primers: Primer 1 5'-GGCAGCTGGT AAAAAAAAA CAAAAAAATC CTAGCAAACC-3' (SEQ ID NO: 3), and Primer 2 (XhoI) 5'-CGCCTCGAGT TATTCGGACA ATGCCACGTC AT -3' (SEQ ID NO: 4). The aprE promoter and aprE signal sequence were independently amplified from the p2JM103BBI expression vector using the following primers: Primer 3 5'-TTGTTTTTTT TTACCAGCTG CCTGCGCGCT CA-3' (SEQ ID NO: 5) and Primer 4 (EcoRI) 5'-CGCGAATTCT CCATTTTCTT CTGCTATC -3' (SEQ ID NO: 6) (Vogtentanz, Protein Expr Purif, 55:40-52, 2007 (Vogtentanz, "Protein Expression Purification", Vol. 55, pp. 40-52, 2007)).

[0325] The two PCR products were then assembled into one by a third PCR reaction using primers 2 and 4. After digestion with EcoRI / XhoI, the PCR product was cloned into the p2JM103BBI expression vector digested with the same restri...

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Abstract

The present compositions and methods relate to an endo-Beta-mannanase cloned from Geobacillus tepidamans, polynucleotides encoding the endo-Beta-mannanase, and methods of use thereof. Formulations containing the endo-Beta-mannanase are highly suitable for use as detergents.

Description

[0001] priority [0002] This patent application claims priority to International Patent Application No. PCT / CN2011 / 073536 filed on April 29, 2011, which is hereby incorporated by reference in its entirety. technical field [0003] The compositions and methods of the present invention relate to endo-beta-mannanases cloned from Geobacillus thermophila, polynucleotides encoding endo-beta-mannanases, and methods of their use. Formulations comprising endo-beta-mannanase are very suitable for use as detergents. Background technique [0004] Current laundry detergent and fabric care compositions include ingredients such as surfactants, enzymes (proteases, amylases, mannanases and / or cellulases), bleaches, builder systems, suds suppressors, soil suspending agents Complex combinations of active ingredients such as , detergents, optical brighteners, softeners, dispersants, dye transfer inhibiting compounds, abrasives, bactericides and fragrances. [0005] Mannanases, including endo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/195C12N9/24A23K1/165A23L1/00
CPCC12N9/2494A23K10/14A23K20/189C11D3/38636C12C5/004C12Y302/01078
Inventor B·E·琼斯M·科尔克曼Z·钱B·S·劳尔森K·M·克拉格S·普莱斯鲁斯Z·于L·M·贝比M·埃斯塔布鲁克L·华
Owner DANISCO US INC
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