Industrialized culture method of high protein desert green algae

A culture method and high-protein technology, applied in the direction of single-cell algae, etc., can solve the problems of unstable separation culture method and low yield of desert green algae

Active Publication Date: 2016-03-02
新疆拓必达科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for industrialized cultivation of high-protein desert green algae, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the problem of existing desert green algae. Cultivation method The problem of unstable separation and cultivation method and low yield of cultivating desert green algae

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  • Industrialized culture method of high protein desert green algae
  • Industrialized culture method of high protein desert green algae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the industrialized cultivation method of this high-protein desert green algae is carried out according to the following steps: the first step, separates, and takes desert soil sample, adds the TAP liquid culture medium of 200ml to 400ml in every 50g to 100g desert soil sample According to the method, TAP liquid medium was added to the desert soil sample, and the shaker was shaken for 5min to 15min at a speed of 100rpm to 120rpm to fully mix the sample with the medium. Cultivate under the condition of 2000Lux to 3000Lux for 3 days to 15 days. After the culture supernatant turns green, apply every 200μL to 300μL supernatant on 25ml to 30ml TAP solid medium, and draw the supernatant under sterile conditions solution, coated in a petri dish containing TAP solid medium, and cultured for 3 to 15 days at a temperature of 25°C to 31°C and a light of 2000Lux to 3000Lux to obtain a culture of desert green algae;

[0021] In the second step, purification, each si...

Embodiment 2

[0023] Embodiment 2, the industrialized cultivation method of this high-protein desert green algae is carried out according to the following steps: the first step, separates, and takes desert soil sample, adds the TAP liquid culture medium of 200ml or 400ml in every 50g or 100g desert soil sample Add TAP liquid medium to the desert soil sample, and shake it with a shaker for 5min or 15min at a speed of 100rpm or 120rpm to fully mix the sample with the medium. Cultivate at 2000Lux or 3000Lux for 3 days or 15 days. After the culture supernatant turns green, apply every 200μL or 300μL of supernatant to 25ml or 30ml of TAP solid medium, and draw the supernatant under sterile conditions solution, coated in a petri dish containing TAP solid medium, and cultured for 3 days or 15 days at a temperature of 25°C or 31°C and a light of 2000Lux or 3000Lux to obtain a culture of desert green algae;

[0024] In the second step, purification, each single desert green algae colony is inocu...

Embodiment 3

[0026] Embodiment 3, preferably as above-mentioned embodiment, TAP liquid culture medium is 0.4gNH 4 Cl, 0.156gMgSO 4 , 0.05gCaCl 2 ·H 2 O, 0.142gK 2 HPO·3H 2 O, 2.42g TrisBase, 1.5ml HCl, 1.0ml glacial acetic acid, 2gCH 3 COONa·3H 2 O and 1 ml of trace elements are dissolved in water to prepare 1 L of solution to obtain a TAP liquid medium, and the pH range of the TAP liquid medium is 6.8 to 7.0.

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Abstract

The invention relates to a method for cultivating microbial algae, which is an industrialized method for cultivating high-protein desert green algae. The method is carried out according to the following steps: the first step is to separate the culture of desert green algae; the second step is to separate a single desert green algae The algal colony is inoculated on a solid plate and cultured repeatedly until the 8th to 10th generations are inoculated to obtain the algae body; in the third step, the algae body is inoculated into the liquid medium, and the industrial airtight culture is carried out for 3 days to 15 days. The present invention firstly cultivates desert green algae, increases the cell concentration in the desert soil culture solution, obtains the target algae species with high biomass in the desert soil culture solution, then separates the target algae species, and then performs purification to obtain the high-yield target algae species After planting, carry out large-scale industrialization cultivation at last, can guarantee the successful separation target algae strain like this, and the cultivation method that the present invention adopts is completely aseptic cultivation, can not be polluted by pollution source and harmful bacteria, while guaranteeing desert green algae output Under certain circumstances, desert green algae can be cultivated on a large scale.

Description

technical field [0001] The invention relates to a method for cultivating microbial algae, which is an industrialized method for cultivating high-protein desert green algae. Background technique [0002] Algae (Algae) is a class of autotrophic phytophytes with photosynthetic pigments in the plant kingdom, and has neither leaves, seeds or roots, nor flowers. Some algae are extremely small, only a few microns, but some are very large, very complex tissues, like broad streamers, such as kelp. Algae reproduce with spores in various shapes and sizes, most of which are primitive plants more than 2 billion years ago. Most algae have existed since ancient times and still exist today. Algae are so adaptable that they can survive even in extremely harsh climatic conditions, such as in salty or icy seas. [0003] As we all know, protein feed refers to beans, cakes, fishmeal, etc. whose natural moisture content is lower than 45%, crude fiber in dry matter is lower than 18%, and crude ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12
Inventor 艾山江阿不都克力木白克尔古丽山买买提
Owner 新疆拓必达科技发展有限公司
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