A method for extracting fly maggot protein by using ceramic membrane

A fly maggot protein and ceramic membrane technology, applied in the field of protein extraction, can solve the problems of low purity and low filtration precision, and achieve the effects of high extraction rate, simple process and high efficiency

Active Publication Date: 2016-07-13
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the protein extracted by the above-mentioned method retains its biological activity, it is all filtered with a filter cloth, and the filtration accuracy is not high. The finished product contains many invalid impurity components, so the purity is not high. Extract protein, but not all proteins can be absorbed by the human body, so it is necessary to develop a method for extracting proteins with high purity and easy absorption by the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take fly maggots, wash them, dry them, and grind them into powder. After mixing 1kg of fly maggot powder and 20kg of water evenly, heat up to 50°C, add 50g of trypsin for hydrolysis, and hydrolyze for 60 minutes. The feed liquid enters the enzyme inactivating device. Heat up to 75°C for 5 minutes to inactivate the enzyme. The hydrolyzate is sent to a sand filter for filtration, and the filtrate is sent to a ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 500nm, and the material is alumina. Adjust the pH of the feed liquid to about 6.0. The pressure difference is 0.05Mpa, the membrane surface flow rate is 1m / s, and the filtration temperature is 40°C. After the feed liquid is concentrated 6 times, the microfiltration is stopped. Send the microfiltration permeate to the ultrafiltration membrane for filtration. The material of the ceramic ultrafiltration membrane is zirconia, the average pore size is 20n...

Embodiment 2

[0024] Take fly maggots, wash them, dry them, and grind them into powder. Mix 1kg of fly maggot powder with 20kg of water evenly, heat up to 50°C, add 50g of trypsin for hydrolysis, and hydrolyze for 60 minutes. The feed liquid enters the enzyme inactivating device. Heat up to 75°C for 5 minutes to inactivate the enzyme. The hydrolyzate is sent to a sand filter for filtration, and the filtrate is sent to a ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 200nm, and the material is alumina. Adjust the pH of the feed liquid to about 6.0. The pressure difference is 0.1Mpa, the membrane surface flow rate is 1m / s, and the filtration temperature is 40°C. After the feed liquid is concentrated by 6 times, the microfiltration is stopped. Send the microfiltration permeate to the ultrafiltration membrane for filtration. The material of the ceramic ultrafiltration membrane is zirconia, the average pore size is 20nm, the...

Embodiment 3

[0026]Take the fly maggots, wash them, dry them, and grind them into powder. Mix 1 kg of fly maggot powder with 30 kg of water evenly, heat up to 50°C, add 50 g of trypsin for hydrolysis, and hydrolyze for 55 minutes. The feed liquid enters the enzyme inactivating device. Heat up to 75°C for 5 minutes to inactivate the enzyme. The hydrolyzate is sent to a sand filter for filtration, and the filtrate is sent to a ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 200nm, and the material is alumina. Adjust the pH of the feed liquid to about 6.0. The pressure difference is 0.3Mpa, the membrane surface flow velocity is 5m / s, and the filtration temperature is 40°C. After the feed liquid is concentrated 8 times, the microfiltration is stopped. Send the microfiltration permeate to the ultrafiltration membrane for filtration. The material of the ceramic ultrafiltration membrane is zirconia, the average pore size is 20...

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Abstract

The invention relates to a method for extracting fly maggot animal protein by ceramic membrane separation technology. Fresh fly maggots are cleaned, broken, heat-treated, enzymatically hydrolyzed, and enzyme-killed, and the enzymolyzed solution is separated and purified by ceramic membrane separation technology. In the steps of concentration and spray drying, in the enzymatic hydrolysis solution, the coarse filter is used to remove large particles, and then the macromolecular colloid and oil are removed by ceramic microfiltration membrane and ultrafiltration membrane respectively, and the decomposed protein is mixed with Peptides and amino acids are separated, and the concentrated solution is spray-dried to obtain protein powder, which is then concentrated and dried by nanofiltration to obtain a mixture of polypeptides, amino acids and small molecular proteins. The method can effectively obtain the fly maggot protein, reduce the bitterness of the extracted product, have high extraction efficiency, and can simultaneously separate polypeptides and amino acid small molecule proteins.

Description

technical field [0001] The invention relates to a method for extracting protein from fly maggots, in particular to a process for extracting fly maggot animal protein by coupling enzymatic hydrolysis and a ceramic membrane method, and belongs to the technical field of biotechnology enzymatic hydrolysis of fly maggot protein and membrane separation. Background technique [0002] With the rapid development of the breeding and feed industries, my country's demand for animal protein is also increasing. At present, the main source of animal protein in my country is fishmeal. Domestic fishmeal cannot meet the market demand, so a large amount of fishmeal needs to be imported every year. The dry base of fly maggots contains 54%~65% of crude protein, 10%~14% of fat, 8%~10% of chitin and rich vitamins, trace elements, etc. The nutritional components are relatively comprehensive, and the total amount of amino acids is 2.3 times that of fish meal. , Methionine and lysine are 2.7 times a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 彭文博张桂花寇琴熊福军张宏范克银
Owner JIANGSU JIUWU HITECH
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