Pneumatic self-circulation animal cell culture bioreactor and application method thereof

A technology of animal cell culture and bioreactor, applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical equipment and methods, etc., can solve the problem of uneven mixing of large tank liquid, cell Cultivate problems such as low pollution rate and affecting product yield, so as to avoid flaws and defects, simplify pipeline layout, and solve the problem of heating overcharge
CN103589638AActive Publication Date: 2014-02-19็ฝ—็ซ็”Ÿ
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
็ฝ—็ซ็”Ÿ
Publication Date
2014-02-19

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Abstract

The invention discloses a pneumatic self-circulation animal cell culture bioreactor and an application method thereof. The bioreactor is characterized by comprising a large tank, a small tank located in the large tank, a magnetic stirring system located at the bottom of the large tank, a heating system and a controlling system, wherein a sealing cover is arranged at the upper end of the large tank, jackets connected with the heating system are arranged on two sides of the large tank, and a highest liquid level sensor of the large tank is arranged at the upper part in the large tank; and a sealing head is arranged at the upper end of the small tank, an opening is formed in the lower end, the sealing head is fixedly connected in a sealing manner with the sealing cover through a breathing pipe, the upper end of the breathing pipe penetrates out of the sealing cover and is connected with a vacuum air pump and an air pressure pump respectively, the lower end penetrates out of the sealing head and enters the small tank, a cavity used for accommodating a cell carrier is defined by two up-and-down horizontal stainless steel wire mesh boards in the middle of the small tank and the inner wall of the small tank, an upper liquid level sensor of the small tank is arranged above the cavity, and a lower liquid level sensor of the small tank is arranged below the cavity. The pneumatic self-circulation animal cell culture bioreactor has the advantages of good air tightness, simplicity in operation, low contamination rate, good cell quality and high yield.
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Description

technical field

[0001] The invention relates to an animal cell culture device, in particular to a pneumatic self-circulation animal cell culture bioreactor and its application method. Background technique

[0002] Bioreactor refers to equipment that uses living cells or enzymes as biocatalysts to provide a suitable environment for cell proliferation or biochemical reactions. It is the key equipment in the process of biological reactions. The selection of the structure, operation mode and operating conditions of the bioreactor is closely related to the quality, yield (conversion rate) and energy consumption of biochemical products. With the continuous development of biotechnology, large-scale animal cell bioreactor culture technology is gradually replacing glass bottle static culture and rotary culture technology, because it can provide the best living environment for the cultured objects in time, so that the most efficient It should be said that this is a qualitative leap i...

Claims

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