Cultivation method of virus-free Diaogua tissue culture seedlings
A culture method and tissue culture seedling technology are applied in the field of virus-free tissue culture seedling cultivation, which can solve problems such as being susceptible to virus infection, and achieve the effects of improving survival rate, ensuring yield and quality, and high yield.
Active Publication Date: 2015-08-12
绍兴市农业科学研究院
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Problems solved by technology
[0004] The purpose of the present invention is to address the above problems, to provide a method for cultivating virus-free Diaogua tissue culture seedlings; to solve the problem that the existing Diaogua tissue culture seedlings in the prior art have not undergone virus-free treatment, and are easily infected by viruses after being transplanted into the field. dyeing technical issues
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[0026] A method for cultivating virus-free Diaogua tissue culture seedlings, comprising the following steps:
[0027] Step 1, obtaining Diaogua tissue culture seedlings in laboratory culture medium;
[0028] Step 2, placing the Diaogua tissue culture seedlings in the light incubator, the temperature of the light incubator is alternately changed every 12 hours in two temperature ranges of 31°C-33°C and 37°C-39°C, and the culture time is 8-12 days;
[0029] Step 3, after the cultivation is completed, take out the Diaogua tissue culture seedlings from the light incubator, strip off the shoot tips of 0.2-0.4mm under the dissecting microscope, and inoculate them in MS+2.0mg·L-1BA+ with a pH of 5.6-6.0 30.0mg·L-1 sucrose + 0.6% agar medium, put the medium into a culture room for cultivation, the temperature of the culture room is 23°C-27°C, the relative humidity is 60%-80%, and the light intensity is 1800 -2200Lx, the light time is 10-14h / d;
[0030] Step 4, when the shoot tip tu...
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The invention provides a culture method of a virus-free snake gourd tissue culture seedling, belonging to the technical field of agricultural planting and solving the technical problem that a snake gourd tissue culture seedling is easily infected by virus after being planted in a field because of not being subjected to virus removal treatment in the prior art. The culture method comprises the following steps: obtaining the snake gourd tissue culture seedling, carrying out temperature-varying culture on the snake gourd tissue culture seedling in an illumination incubator for 8-12 days, carrying out inoculated culture on stem tips after culture is completed, and transferring the inoculated culture to obtain a virus-free plant in a multiplication step. According to the culture method, the virus-free snake gourd tissue culture seedling is obtained; and the virus-free snake gourd tissue culture seedling is not easily infected by the virus after being transplanted to the field, is good in consistency, strong in growth and high in yield, and ensures the yield and quality of snake gourd seeds.
Description
technical field [0001] The invention belongs to the technical field of agricultural planting, and relates to a method for cultivating virus-free hanging melon tissue culture seedlings. Background technique [0002] Diaogua (Fructus Trichosanthis Kirilowii) is a perennial herbaceous climbing plant of Angiospermae, Dicotyledones, Cucurbitaceae, and Trichosanthes. It can be planted in eastern my country, the Yangtze River and the Yellow River Basin. Diaogua fruit is white and light green or dark green pattern, orange-red when ripe. The edibility development of Diaogua mainly focuses on Diaogua seeds. Diaogua seeds account for more than one-third of the net weight of Diaogua fruit. Generally, Diaogua fruit contains 75-210 seeds, and the yield of melon seeds per mu is 75-150 kg. Modern medical research proves that fatty acids in hanging melon seeds account for 36.2%, of which 65% are unsaturated fatty acids, protein accounts for 5.46%, and contains more than 17 kinds of amino a...
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IPC IPC(8): A01H4/00
Inventor 孙叶芳郑琪羊安兴邢海赵虎黄岳夫
Owner 绍兴市农业科学研究院