Application of arabidopsis thaliana At5g61350 gene to aspect of root system development

Abstract

The invention belongs to the field of genetic engineering, and particularly relates to application of an arabidopsis thaliana At5g61350 gene to the aspect of root system development. A protein coded by the arabidopsis thaliana At5g61350 gene regulates and controls the transport of NH4<+>, and a gene mutant, namely, an arabidopsis thaliana missing the At5g61350 gene or silencing the At5g61350 gene does not tolerate to an ammonium salt. In the prior art, although the prediction of the At5g61350 gene is related to the growth and development of a plant root system, precise functions about the gene are always not confirmed because of being lack of related experiment proofs. An inventor proves that through a series of experiments: the arabidopsis thaliana At5g61350 gene is related to the transport of the NH4<+>, and involves in the path of controlling the root hair growth through regulating and controlling the concentration of Ca<2+> at the tips of root hairs so that the development and growth of the root system are regulated and controlled, and thus new possibility is provided for culturing a new crop strain by using the gene.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to Arabidopsis At5g61350 Application of genes in regulating plant root development. Background technique [0002] Nitrogen is one of the most important nutrient elements for plants, accounting for about 1%-5% of the dry weight of plants, of which nitrate and ammonium salts are the main sources of nitrogen nutrition for plants to obtain from the soil. Ammonium (ammonium, NH 4 + ) As one of the main providers of nitrogen nutrition, plants tend to preferentially absorb ammonium ions because they consume less energy than nitrate ions when assimilating ammonium ions. At the same time, ammonium ion, as an important signal molecule, participates in the regulation of many physiological processes, so it is of great significance to study the transport and signal transduction of ammonium ion in plant cells. [0003] Arabidopsis At5g61350 The gene belongs to the CrRLK1L (Receptor like ki...

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Problems solved by technology

[0003]Arabidopsis At5g61350 gene belongs to CrRLK1L (Receptor like kinase) receptor protein kinase gene, its CDS length is 2529bp, encoding 842 Amino acid protein, studies have shown that it is related to the development of root hairs in plants, but there is no research report on the relationship between this gene and NH4+ translocation, and thus Mediated root growth has not been reported

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