Application of Arabidopsis at5g61350 Gene in Root Development

Abstract

The invention belongs to the field of genetic engineering, in particular to Arabidopsis At5g61350 Application of genes in regulating plant root development. Arabidopsis At5g61350 The protein encoded by the gene regulates the transport of NH4+, and the mutant of the gene, that is, the deletion At5g61350 gene or At5g61350 Gene-silenced Arabidopsis is intolerant to ammonium salts. In the prior art, although predicting At5g61350 The gene is related to the growth and development of plant roots, but due to the lack of relevant experimental evidence, the exact function of the gene has not been confirmed. The inventor proved through a series of experiments that Arabidopsis At5g61350 The gene is related to NH4+ transport, and participates in the pathway that NH4+ controls root hair growth by regulating the Ca2+ concentration gradient at the tip of the root hair to regulate the development and growth of the root system, thus providing a new possibility for using this gene to cultivate new crop lines.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to Arabidopsis At5g61350 Application of genes in regulating plant root development. Background technique [0002] Nitrogen is one of the most important nutrient elements for plants, accounting for about 1%-5% of the dry weight of plants, of which nitrate and ammonium salts are the main sources of nitrogen nutrition for plants to obtain from the soil. Ammonium (ammonium, NH 4 + ) As one of the main providers of nitrogen nutrition, plants tend to preferentially absorb ammonium ions because they consume less energy than nitrate ions when assimilating ammonium ions. At the same time, ammonium ion, as an important signal molecule, participates in the regulation of many physiological processes, so it is of great significance to study the transport and signal transduction of ammonium ion in plant cells. [0003] Arabidopsis At5g61350 The gene belongs to the CrRLK1L (Receptor like ki...

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Problems solved by technology

[0003]Arabidopsis At5g61350 gene belongs to CrRLK1L (Receptor like kinase) receptor protein kinase gene, its CDS length is 2529bp, encoding 842 Amino acid protein, studies have shown that it is related to the development of root hairs in plants, but there is no research report on the relationship between this gene and NH4+ translocation, and thus Mediated root growth has not been reported

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