A method for increasing the yield of influenza virus vaccine strains

Abstract

The invention provides a method for increasing the yield of an influenza virus vaccine strain. According to the method, yield of the influenza virus vaccine strain in cell production is increased through overexpression of an FMRP protein or a functional truncated protein containing a KH2 structural domain in cells used for production of the influenza virus vaccine strain. Results show that replication efficiency of influenza viruses is increased by more than 2 times through transient or stable expression of the FMRP protein in cells (such as Vero and MDCK cells) used for production of the influenza virus vaccine strain, and vaccines are prepared from the obtained viruses so as to meet market demands for influenza vaccines.

Description

technical field [0001] The invention relates to the preparation of influenza virus vaccine strains, in particular to a method for increasing the yield of influenza virus vaccine strains. Background technique [0002] Influenza (flu) is one of the most common infectious diseases in humans. Frequent regional outbreaks and uncertain influenza pandemics seriously endanger human health and cause huge economic losses. Because influenza virus is prone to antigenic mutation, gene recombination, and drug-resistant strains, influenza vaccination has become one of the necessary measures to control seasonal influenza epidemics and influenza pandemics. [0003] Traditional influenza vaccine production is mostly done in fertilized eggs between 9 and 12 days old. In 2007, the European Union approved the marketing of Novartis' influenza vaccine produced in mammalian cells. The production of influenza vaccine in mammalian cells has greater scalability than that in eggs, and can eliminate th...

AI Technical Summary

Problems solved by technology

However, at this stage, due to the limitation of production technology, the yield of using cells to produce influenza vaccines is low, which greatly limits the development and utilization of influenza vaccines, especially affecting the rapid production and vaccination of emergency vaccines during influenza pandemics

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