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A method for localization of quantitative trait loci in seeds based on mixed linear models

A hybrid linear model, seed number technology, applied in the fields of electrical digital data processing, special data processing applications, instruments, etc., can solve the unreported environmental effects and gene-environment, no epistasis and gene-environment interaction effects Integrate other problems in a model to achieve the effect of improving efficiency and accuracy

Active Publication Date: 2016-08-17
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

[0007] However, so far, neither environmental effects nor gene-environment interaction effects have been reported, nor have methods to integrate epistasis and gene-environment interaction effects into one model

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  • A method for localization of quantitative trait loci in seeds based on mixed linear models
  • A method for localization of quantitative trait loci in seeds based on mixed linear models
  • A method for localization of quantitative trait loci in seeds based on mixed linear models

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Embodiment Construction

[0063] The present invention will be further explained below in conjunction with specific examples.

[0064] The invention divides the seeds into triploid seeds and diploid seeds based on the method for locating the quantitative trait loci of seeds based on the mixed linear model.

[0065] 1. If the trait being analyzed is primarily controlled by maternal and endosperm genetic effects, methods include:

[0066] (1) Establishment of statistical genetic model:

[0067] According to the characteristics of the seeds and the purpose of the experiment, an appropriate statistical genetic model of the seed traits is established. For a mating mapping population derived from two homozygous parents (P1, P2), it is assumed that genetic experiments are carried out in t different environments, and b blocks are set in each environment. The variation of a certain seed trait is affected by s QTL (Q 1 , Q 2 ,...,Q s ) and t epistasis regulation, then the observed value of the seed trait ph...

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Abstract

The invention discloses a method for locating seed quantitative trait loci based on a mixed linear model, comprising: establishing a statistical genetic model; searching for all candidate marker intervals that may have QTL in the whole genome; using the candidate marker intervals as a collaborative Variables, search for significant QTL sites and two-interaction marker intervals in the whole genome; use the QTL sites and two-interaction marker intervals as covariates, search for significant two-interaction marker intervals within the significant interaction marker interval The site of epistasis; through the QTL site and the site of two interaction epistasis, obtain the coefficients of various effects in the statistical genetic model, and then use the statistical genetic model to calculate the effect of these sites and estimate each The heritability of the locus. The invention divides the seed traits into diploid seeds and triploid seeds, considers the epistasis effect and the interaction effect between genes and the environment, and can quickly and unbiasedly estimate the position of the seed quantity trait locus and various effects.

Description

technical field [0001] The invention relates to the field of location of quantitative traits of seeds, in particular to a method for location of quantitative traits of seeds based on a mixed linear model. technical background [0002] Crop seeds are a major source of human food, animal feed, and industrial raw materials. The embryo or endosperm is the main component in the seed. The endosperm is the main organ that stores nutrients, such as carbohydrates, proteins and minerals, and it also provides nutrients for the development of the embryo. The endosperm disappears as some species mature, such as Arabidopsis. Regardless of whether the endosperm disappears or not, it will have an impact on the development of the embryo. In addition, the seeds are grown in the mother plant, so the mother plant will also have an impact on the development of the seeds. The study on the location of quantitative trait loci in seeds has become one of the research hotspots in the field of quan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/12
Inventor 徐海明祁婷曹玉杰祝水金楼向阳
Owner ZHEJIANG UNIV