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A kind of artificial seedling raising method of partridge

A partridge and artificial technology, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as uncontrollable spore development ability, uneven seedlings, and inaccurate control of spore density , to optimize the layout density, increase the biomass, and shorten the growth cycle

Active Publication Date: 2016-03-23
中国科学院汕头海洋植物实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development ability of the spores collected in the traditional way is uncontrollable, which may lead to unevenness in the developed seedlings
Furthermore, the spore density on the attachment cannot be precisely controlled, so that the place where the spores are attached more will grow lushly, and the place where the spores will be attached less will grow sparsely.

Method used

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  • A kind of artificial seedling raising method of partridge
  • A kind of artificial seedling raising method of partridge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Sterilize the washed partridge in a solution containing penicillin sodium 25TU / L for 25 minutes, cut into 1.2 mm partridge tissue pieces under a dissecting microscope, rinse with sterile water and transfer to a sieve for culturing Keep the solution moist for 3 hours; put the sieve silk attached to the partridge tissue block into the incubator for cultivation, the cultivation temperature is 20°C, the light intensity is 1500Lux, the photoperiod is 12:12 hours, and the dew is dried for 4 hours every day, every four days Replace the culture medium once, and after 15 days of cultivation, the seedlings are moved to the sea area for stocking.

[0030] Use sterilized seawater and distilled water to adjust the culture medium to a salinity of 6.5‰ (specific gravity 1.005), add NaNO 3 Make its concentration in the culture solution be 6ppm (mass ratio, the same below), add KH 2 P0 4 Make the concentration in the culture solution 0.6ppm, add 6-benzylaminopurine to make the concent...

Embodiment 2

[0032] Sterilize the washed partridge in a solution containing penicillin sodium 25TU / L for 25 minutes, cut it into 1.2 mm tissue pieces under a dissecting microscope, rinse with sterile water and move to a sieve to keep it moist with culture medium 3 hours. Put the sieve silk attached to the tissue block into the incubator for cultivation. The cultivation temperature is 25°C, the light intensity is 2000Lux, the photoperiod is 12:12 hours, and the dew is dried for 5 hours every day. The culture medium is replaced every four days. The seedlings are moved to the sea area for stocking.

[0033] Use sterilized seawater and distilled water to adjust the culture medium to a salinity of 12.8‰ (specific gravity 1.010), add NaNO 3 Make its concentration in the culture solution be 8ppm (mass ratio, the same below), add KH 2 P0 4Make the concentration in the culture solution 0.8ppm, add 6-benzylaminopurine to make the concentration in the culture solution 0.06ppm, add dichlorophenoxya...

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Abstract

The invention relates to an artificial breeding method for caloglossa leprieurii. The artificial breeding method comprises the following steps: selecting eugonic caloglossa leprieurii and removing attached matters and wild seaweeds on the surfaces of algae; sterilizing in a solution containing 20-60TU / L penicillin sodium for 20-40 minutes; cutting the caloglossa leprieurii into tissue blocks with the size of 1mm-3mm and transferring the tissue blocks to 200-300-mesh bolting cloth; adding a culture solution and drying at least for 3-5 hours each day in the open under the conditions that the temperature is 20-25 DEG C, the illumination intensity is 1500Lux-2000Lux and the illumination period is 12:12 hours; replacing the culture solution for one time every 4-5 days; washing the algae by using sterile distillation water during replacing of the culture solution; and inoculating for 15-20 days so as to obtain germchits. According to the artificial breeding technology for the caloglossa leprieurii, the growth cycle can be greatly shortened and the germchits can be produced all the year round; the cultivation frequency is increased and the yield of the caloglossa leprieurii is continually increased. The requirements of production are met and the requirements of patients are met; the artificial breeding method for the caloglossa leprieurii has great social benefits.

Description

technical field [0001] The invention relates to an artificial seedling raising method of partridge tissue culture. Background technique [0002] Partridge Caloglossaleprieurii (Montagne) J.I belongs to the order Ceramiales and the family Delesseriaceae. It is a warm-temperate seaweed that is distributed in Zhejiang, Fujian and the coastal areas of Guangdong in my country. Because partridge contains degenic acid and degeneaside, which have anthelmintic effect, it is used by traditional Chinese medicine to treat roundworms parasitic in the human large intestine. The Chinese patent medicine compound partridge powder is based on partridge The dish is made from the main raw material. It is also often added to some traditional Chinese medicine formulas for diseases caused by insect accumulation or insect bites. Such as the treatment of acute pancreatitis of insect accumulation type, the treatment of insect accumulation type insomnia and the treatment of insect bite type ecchymos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈加雄李伟滨林传旭马庆涛陈楷亮
Owner 中国科学院汕头海洋植物实验站
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