Method for continuously extracting L-tryptophan from fermentation liquor

A technology of tryptophan and fermentation liquid, which is applied in animal feed, animal feed, additional food elements, etc., can solve the problems of yield drop, quality drop, tryptophan discoloration, etc., and achieve high yield and low mother liquor volume Effect

Active Publication Date: 2014-03-19
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent still adopts the traditional heating concentration method in the later stage of concentration, and there is a prob

Method used

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Examples

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Effect test

Embodiment 1

[0024] The method of the present invention (Example 1) for continuously extracting L-tryptophan from the fermentation broth comprises the following steps:

[0025] 1000L of L-tryptophan fermentation broth with a concentration of 40.18g / L was adjusted to a pH value of 4.1 with hydrochloric acid in a fermenter, and heated to inactivate to form a feed liquid;

[0026] The feed liquid enters the high-speed tubular centrifuge to remove bacteria and precipitated insoluble protein to form a centrifugal effluent;

[0027] Use a 3000 Dalton membrane to remove most of the pigment in the centrifuged effluent, and use a 200 Dalton tubular nanofiltration membrane system to nanofilter the decolorized effluent until the feed liquid is slightly cloudy to form a concentrate, and the tubular nanofiltration membrane system maintains The temperature is 45°C;

[0028] The concentrated solution is cooled and crystallized by a tubular crystallizer to form a crystallization solution. The crystalli...

Embodiment 2

[0032] The method of the present invention (Example 2) for continuously extracting L-tryptophan from the fermentation broth comprises the following steps:

[0033] 2500L of L-tryptophan fermentation broth with a concentration of 39.97g / L was adjusted to pH 4.2 with hydrochloric acid, and heated to inactivate to form feed liquid;

[0034] Use compressed air to hydraulically feed the material into a high-speed tubular centrifuge to remove bacteria and precipitated insoluble protein to form a centrifuge effluent;

[0035] The centrifuged effluent enters the feed liquid storage tank of the decolorization membrane system, and a 3000 Dalton membrane is used to remove most of the pigments, and the decolorized effluent enters the feed liquid storage tank of the 200 Dalton tubular nanofiltration membrane system, and the nanofiltration Until the feed liquid is slightly muddy to form a concentrate, the tubular nanofiltration membrane system maintains a temperature of 45°C;

[0036] The ...

Embodiment 3

[0040] The method of the present invention (Example 3) for continuously extracting L-tryptophan from the fermentation broth comprises the following steps:

[0041] 2000L of L-tryptophan fermentation broth with a concentration of 40.02g / L was adjusted to a pH value of 3.8 with hydrochloric acid in a fermenter, and heated to inactivate to form a feed liquid;

[0042] The feed liquid enters the high-speed tubular centrifuge to remove bacteria and precipitated insoluble protein to form a centrifugal effluent;

[0043] Use a 3000 Dalton membrane to remove most of the pigment in the centrifuged effluent, and use a 200 Dalton tubular nanofiltration membrane system to nanofilter the decolorized effluent until the feed liquid is slightly cloudy to form a concentrate, and the tubular nanofiltration membrane system maintains The temperature is 45°C;

[0044] The concentrated solution is cooled and crystallized by a tubular crystallizer to form a crystallization solution. The crystalli...

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Abstract

The invention discloses a method for continuously extracting L-tryptophan from a fermentation liquor. The method comprises the following steps: step 1, regulating the pH value of the fermentation liquor to the range from 3.8 to 4.5 by using hydrochloric acid in a fermentation tank, and heating for inactivation to form a feed liquor; step 2, feeding the feed liquor into a high-speed tube centrifuge, and removing bacteria and insoluble protein separated out to form a centrifugal effluent; step 3, removing most pigment from the centrifugal effluent by using a membrane of 3000 Daltons, and feeding the decolored effluent into a tubular nanofiltration membrane system of 200 Daltons for nanofiltration until the feed liquor is slightly turbid, and then forming a concentrated liquor; step 4, quickly cooling and crystallizing the concentrated liquor through a tubular crystallizer, and subjecting the crystallized liquor to solid-liquid separation to obtain crude tryptophan; step 5, dissolving the crude tryptophan in hot pure water, and adding the hydrochloric acid to regulate the pH value to the range from 4.0 to 5.0 for helping solubilization; decoloring by using activated carbon, and then treating the decolored liquor by the circulating methods from the step 2 to the step 4 in the same way, and finally, drying to obtain white refined tryptophan. The method provided by the invention has the advantage that the quality and yield of the product are improved.

Description

technical field [0001] The invention relates to extraction, in particular to a method for continuously extracting L-tryptophan from fermentation broth. Background technique [0002] Tryptophan has been widely used in medicine, food, feed, etc. The production technology of fermentation method has made great progress, and the extraction technology has also been improved and improved. In the stage of heating and evaporating to remove water, even if some patents adopt membrane system treatment, they only use nanofiltration as the initial concentration of concentration in the early stage, and then transfer to the traditional decompression and heating to evaporate and remove water to crystallization until the concentration treatment is completed. The reprocessing of mother liquor also adopts the same traditional method. [0003] The Chinese patent with the patent number CN101717360A discloses the use of ultrafiltration membranes to remove bacteria and proteins, activated carbon d...

Claims

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Application Information

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IPC IPC(8): C07D209/20A23K1/16
CPCA23K20/00C07D209/20
Inventor 朱燕王志伟张华峰丁亚波曹云锋田学国
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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