Method and device for distinguishing magnetic effects of characterizing materials of fluorescence spectrum through time
A technology of time-resolved fluorescence and fluorescence spectroscopy, which is applied in the field of using time-resolved fluorescence spectroscopy to characterize the magnetic effect of substances, can solve the problems of inability to detect the response of biochemical magnetic sensitive substances, etc., and achieve the effect of simple operation, simple principle and small demand
Active Publication Date: 2014-03-19
NAT UNIV OF DEFENSE TECH
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[0005] The technical problem to be solved by the present invention is: aiming at the technical problems existing in the prior art, the present invention provides a method with simple principle, easy operation, and the ability to use time-resolved fluorescence spectroscopy to characterize the magnetic effects of proteins and other magnetically sensitive substances. The method and device solve the problem of inability to detect the response of biochemical magnetosensitive substances to magnetic fields
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[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0031] Because most biological tissues have fluorescent structures, in order to enrich the characterization methods of the magnetic effects of biological samples, the present invention designs a method and device for characterizing the magnetic effects of samples using fluorescence spectroscopy. Fluorescence is a type of radiative transition, which is the radiation released when an excited state molecule deactivates to a lower energy state where the multiplicity does not change. The commonly used excitation light is ultraviolet or X-ray, and the emitted fluorescence is generally longer than the wavelength of the incident light, and is distributed in the visible light band. Once the excitation light is stopped, the luminescent phenomenon disappears immediately.
[0032] According to the mechanism of free radical pair, the material wit...
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Disclosed are a method and a device for distinguishing magnetic effects of the characterizing materials of a fluorescence spectrum through time. The method comprises the following steps of (1) determining the laser wavelength of the fluorescence spectrum; (2) determining a fluorescence detecting wavelength; (3) putting a sample; (4) according to the testing result of the step (1), generating pulse laser with the wavelength of lambda ex; (5) exciting magneto-sensitive materials to generate fluorescent light through the pulse laser; (6) converting the fluorescent light into electric signals through a photomultiplier after the fluorescent light is split; (7) monitoring the pulse laser signals and the photomultiplier signals to obtain the time-distinguished dynamic fluorescence spectrum of the sample; (8) adjusting the intensity of a magnetic field and repeating the above steps to obtain a series of time-distinguished dynamic fluorescence spectrum; (9) taking out the sample and measuring the central area magnetic field intensity of the position where the sample in disposed; (10) performing multistage fitting on a fluorescence curve and estimating the magnetic effects according to the service life change of the fluorescence light. The device is used for implementing the method above. The method and the device for distinguishing the magnetic effects of the characterizing materials of the fluorescence spectrum through time have the advantages of being simple in principle, easy to operate, good in effects and the like.
Description
technical field [0001] The invention mainly relates to the technical field of characterizing the magnetic effect of matter, in particular to a method and a device for characterizing the magnetic effect of matter by using time-resolved fluorescence spectroscopy. Background technique [0002] All substances are magnetic, but the magnetism of different substances is very different, some substances have strong magnetism, and some substances have weak magnetism. The magnetism of matter will generate a magnetic field in its surrounding space, so it can be further concluded that there is a magnetic field in any space, but some places have strong magnetic fields and some places have weak magnetic fields. This shows that magnetism and magnetic phenomena have universality and extremely rich connotations. Because of this, magnetism and magnetic phenomena are used in an extremely wide range of applications. For example, the biomagnetic effect is one of the active fields in the current...
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Inventor 王璟吴文健杜现礼潘炜松李东晓王孝杰王晓峰
Owner NAT UNIV OF DEFENSE TECH