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A kind of method of producing paeoniflorin

A technology of paeoniflorin and paeoniflorin, which is applied in the field of production of paeoniflorin, can solve problems such as paeoniflorin content and yield research, avoid heavy metal pollution and pesticide residues, benefit society and economic value, and facilitate large-scale production Effect

Active Publication Date: 2015-08-12
NINGBO LIWAH PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, this method has not been reported by any patent or research literature, and the current technology about the culture of peony callus is also mainly focused on callus induction and plant regeneration, and there is no information about paeoniflorin in callus. Studies on the content and yield of

Method used

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  • A kind of method of producing paeoniflorin
  • A kind of method of producing paeoniflorin
  • A kind of method of producing paeoniflorin

Examples

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Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Cultivation of Paeoniae officinalis callus

[0053] (1) Disinfection of explants

[0054] Cut the leaves of peonies cultivated in the laboratory (including two varieties of "Po stick" and "Xianxian", originating in Bozhou, Anhui), soak and rinse in the diluted detergent solution for 20 minutes, and then rinse with tap water. Blot the water on the leaves with absorbent paper, disinfect with 75% alcohol for 30 seconds in an ultra-clean workbench, soak in 10% NaClO for 10 minutes, rinse with sterile water for 5 times, and finally dry the leaves with sterilized filter paper.

[0055] (2) Induction culture of callus

[0056] Cut the sterilized and washed leaves into about 1cm 2 , inserted into the induction medium for induction culture, the culture temperature is 24°C, the daily light time is 14h, the light intensity is about 1500lx, and the culture period is 25 days. The callus induction rates of the leaves of the two varieties of Paeoniae Alba were greater tha...

Embodiment 2

[0060] Example 2 Culture of Paeoniae officinalis stem callus

[0061] (1) Disinfection of explants

[0062] Cut the stems of peonies cultivated in the laboratory (including the two varieties of "Poobang" and "Xianxian", originating in Bozhou, Anhui), soak and rinse them in detergent diluent for 20 minutes, then rinse them with tap water; absorb them with absorbent paper The water on the dry stems was disinfected with 75% alcohol for 30 seconds in an ultra-clean workbench, soaked in 10% NaClO for 10 minutes, rinsed with sterile water for 5 times, and finally the water on the stems was blotted dry with sterile filter paper.

[0063] (2) Induction culture of callus

[0064] The sterilized and washed stems were cut into 0.3-0.5 cm stem segments, inserted into the induction medium for induction culture, the culture temperature was 24°C, the daily light time was 14 hours, the light intensity was about 1500lx, and the culture period was 25 days. The stem induction rate of the peony...

Embodiment 3

[0068] Example 3 Cultivation of Peony Petiole Callus

[0069] (1) Disinfection of explants

[0070] Cut the petioles of peonies cultivated in the laboratory (including the two varieties of "Po stick" and "Xianxian", the place of origin in Bozhou, Anhui), soak and rinse with detergent diluent for 20 minutes, and then rinse with tap water. Blot the water on the petiole with absorbent paper, disinfect it with 75% alcohol for 30 seconds in an ultra-clean workbench, then soak it in 10% NaClO for 10 minutes, rinse it with sterile water for 5 times, and then use sterilized filter paper to dry the water on the petiole.

[0071] (2) Induction culture of callus

[0072] The sterilized and washed petioles were cut into 0.3-0.5 cm, inserted into the induction medium for induction culture, the culture temperature was 24°C, the light time was 14 hours per day, the light intensity was about 1500 lx, and the culture period was 25 days. The induction rates of petiole of two kinds of peonies ...

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Abstract

The invention discloses a method for producing paeonia lactiflora double glycosides, which comprises the following steps: explants are inoculated to an induction medium for induction to produce callus tissues after being sterilized; the callus tissues are inoculated to a proliferation culture medium and are subcultured under the illumination condition, and the paeonia lactiflora double glycosides are extracted from the callus tissues after the subculturing operation is completed. The content of paeonia lactiflora double glycosides cultured in the callus tissues through the method is equal to or greater than that of paeonia lactiflora double glycosides of cultivated paeonia lactiflora, the storage time of the paeonia lactiflora double glycosides is shortened to 30-60 days from 2-4 years, and the pollution of harmful substances such as pesticide residues and heavy metal to soil, water and the like can be avoided during the whole production process. Therefore, the production time can be remarkably reduced, the production cost is reduced, and the produced paeonia lactiflora double glycosides have relatively high safety through the adoption of the method for producing the paeonia lactiflora double glycosides.

Description

technical field [0001] The invention relates to the technical field of natural medicine extraction, in particular to a method for producing paeoniflorin. Background technique [0002] Peony (Paeonia lactiflora Pall.) is a perennial herbaceous plant of the genus Paeonia (Paeonia) in the family Paeoniaceae. It has a long history of cultivation and has been loved by people since ancient times. It is known as "flower phase". [0003] Peony is not only an ornamental plant, but also a medicinal plant. The roots of peony can be used as medicine. Those who directly dry after harvesting are called Radix Paeoniae Alba. Radix Paeoniae Rubra has the functions of clearing away heat and cooling blood, dissipating blood stasis and relieving pain. It is used to treat hot blood entering the camp, warm toxins and spots, vomiting blood and epistaxis, red eyes and swelling pain, liver depression and hypochondriac pain, amenorrhea dysmenorrhea, abdominal pain in the abdomen, and injury from fal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 冯定军鲁俞江江海龙俞伟梅乐和胡升代冬梅马莹莹
Owner NINGBO LIWAH PHARM CO LTD