Acremonium acremonium, its cultivation method and its application in the preparation of root-knot nematode insecticide spore powder

A technology of Acremonium and a cultivation method, applied in the field of microorganisms, can solve problems such as limited effect, soil environment pollution, aggravation of crop diseases, etc., and achieves convenient crushing and sieving, spore growth, and mycelium germination. Effect

Active Publication Date: 2015-12-02
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to direct infection damage, root-knot nematodes also cause compound infection with other pathogens, which aggravates crop diseases and brings great harm to Shaanxi agricultural production
[0004] There are many methods for the control of crop root-knot nematode diseases, but most of them are not very effective
Field rotation and the use of non-host plants or disease-resistant plants are the main management measures to control root-knot nematodes, but they have certain limitations. For example, most species of root-knot nematodes have a wide host range. Most of the populati...

Method used

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Examples

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preparation example Construction

[0049] The preparation method of root-knot nematode insecticide spore former powder is realized by the following steps:

[0050] Step 1: After the liquid fermentation medium was sterilized at 121°C for 30 minutes, inoculated with Acremonium acremonium JXC224, cultured at 28°C±1°C and 180 rpm for 3 days to obtain a seed liquid.

[0051] In step 1, the formula of liquid fermentation medium is:

[0052] Colloidal chitin 1.0-2.0g, glucose 0-1.0g, potassium dihydrogen phosphate 1.0g, calcium chloride 0.2g, sodium chloride 0.2g, magnesium sulfate 0.5g, dilute to 1.0L water.

[0053] Step 2: In addition, the liquid fermentation medium described in 7L step one is placed in a 10L fermenter, and the seed liquid obtained in step one is inoculated in the liquid fermentation medium with an inoculum size of 15% by volume, and stirred at 150 rpm minutes, control dissolved oxygen 0.20-0.28M 3 / M 3 min, liquid submerged fermentation for 72 hours to obtain seed mother liquor.

[0054] Step ...

Embodiment 1

[0059] The root-knot nematode insecticide spore former powder is prepared by the following method:

[0060] (1) Liquid culture:

[0061] Weigh colloidal chitin 2.0g, potassium dihydrogen phosphate 1.0g, calcium chloride 0.2g, sodium chloride 0.2g, magnesium sulfate 0.5g, dissolve in 1000ml distilled water to prepare a liquid fermentation medium, adjust the pH to 7.0, 100ml per bottle was divided into 500ml Erlenmeyer flasks, sterilized at 121°C for 30 minutes, picked and inoculated with Acremonium acremonium JXC224, kept at 27°C, and cultured at 180 rpm for 3 days to make seed liquid.

[0062] In addition, put 7L of the above-mentioned liquid fermentation medium in a 10L fermenter, inoculate the seed liquid in the liquid fermentation medium at a volume ratio of 15%, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, keep at 27°C, and stir for 150 RPM, control dissolved oxygen 0.20M 3 / M 3 min, submerged liquid fermentation for 72 hours to obtain seed mother liquor.

...

Embodiment 2

[0068] A root-knot nematode biocontrol insecticide spore former powder, prepared by the following method:

[0069] (1) Liquid culture:

[0070] Weigh colloidal chitin 1.0g, glucose 1.0g, potassium dihydrogen phosphate 1.0g, calcium chloride 0.2g, sodium chloride 0.2g, magnesium sulfate 0.5g, dissolve in 1000ml distilled water, make liquid fermentation medium, adjust The pH is 7.0, 100ml per bottle is divided into 500ml Erlenmeyer flasks, sterilized at 121°C for 30 minutes, picked and inoculated with Acremonium acremonium JXC224, kept at 28°C, and incubated at 180 rpm for 3 days to make seeds liquid.

[0071] In addition, put 7L of the above-mentioned liquid fermentation medium into a 10L fermenter, inoculate the seed liquid into the liquid fermentation medium at a volume ratio of 15%, adjust the pH to 7.0, sterilize at 121°C for 30 minutes, keep at 28°C, and stir 150 rpm, control dissolved oxygen 0.24M 3 / M 3 min, submerged liquid fermentation for 72 hours to obtain seed m...

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Abstract

The invention relates to acremonium, a culturing method of the acremonium and an application of the acremonium to preparation of original spore powder of a meloidogyne pesticide. Currently, a pollution-free sustainable control method with biological control as the core is used as a main direction of control of meloidogyne diseases. According to the application, the acremonium JXC224 is selected and injected into a liquid fermentation medium, a seed solution is obtained and injected into a solid medium with the inoculation amount of 10% by mass ratio, solid yeast is prepared and dried till the moisture content is lower than 15%, the solid yeast is ground by a dust-collection grinder and screened on a vibrating screen, and the acremonium JXC224 original spore powder is obtained and used as the meloidogyne pesticide. The microbial pesticide original spore powder containing acremonium spores playing a role in infection, killing and biological control of meloidogyne is provided and applicable to the biological control of the serious meloidogyne diseases in vegetable and fruit planting processes of agricultural facilities, and the control effect on the meloidogyne of peppers, tomatoes, cucumbers and the like can exceed 93%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to acremonium acremonium, its cultivation method and its application in the preparation of root-knot nematode insecticide spore powder. Background technique [0002] Root-knot nematode is an obligate endoparasite on plant roots and is the main pathogen that threatens agricultural production worldwide. According to relevant research, root-knot nematode hosts have exceeded 3,000 species of plants, belonging to 114 families, including monocotyledonous plants, dicotyledonous plants, herbaceous plants and gramineous plants. The occurrence of this disease will cause severe crop yield loss, generally resulting in a 10% to 20% yield reduction, and severe cases can reach more than 75%. According to incomplete statistics, nematode damage reduces the world's agricultural output by an average of 24.5% per year, and the economic loss of crops is about 100 billion US dollars. In my...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 沈卫荣孙晓宇路鹏鹏韩丽萍沈俭门欣陈锐
Owner MICROBIOLOGY INST OF SHAANXI
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