Acremonium acremonium, its cultivation method and its application in the preparation of root-knot nematode insecticide spore powder

Abstract

The invention relates to acremonium, a culturing method of the acremonium and an application of the acremonium to preparation of original spore powder of a meloidogyne pesticide. Currently, a pollution-free sustainable control method with biological control as the core is used as a main direction of control of meloidogyne diseases. According to the application, the acremonium JXC224 is selected and injected into a liquid fermentation medium, a seed solution is obtained and injected into a solid medium with the inoculation amount of 10% by mass ratio, solid yeast is prepared and dried till the moisture content is lower than 15%, the solid yeast is ground by a dust-collection grinder and screened on a vibrating screen, and the acremonium JXC224 original spore powder is obtained and used as the meloidogyne pesticide. The microbial pesticide original spore powder containing acremonium spores playing a role in infection, killing and biological control of meloidogyne is provided and applicable to the biological control of the serious meloidogyne diseases in vegetable and fruit planting processes of agricultural facilities, and the control effect on the meloidogyne of peppers, tomatoes, cucumbers and the like can exceed 93%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to acremonium acremonium, its cultivation method and its application in the preparation of root-knot nematode insecticide spore powder. Background technique [0002] Root-knot nematode is an obligate endoparasite on plant roots and is the main pathogen that threatens agricultural production worldwide. According to relevant research, root-knot nematode hosts have exceeded 3,000 species of plants, belonging to 114 families, including monocotyledonous plants, dicotyledonous plants, herbaceous plants and gramineous plants. The occurrence of this disease will cause severe crop yield loss, generally resulting in a 10% to 20% yield reduction, and severe cases can reach more than 75%. According to incomplete statistics, nematode damage reduces the world's agricultural output by an average of 24.5% per year, and the economic loss of crops is about 100 billion US dollars. In my...

AI Technical Summary

Problems solved by technology

In addition to direct infection damage, root-knot nematodes also cause compound infection with other pathogens, which aggravates crop diseases and brings great harm to Shaanxi agricultural production

[0004] There are many methods for the control of crop root-knot nematode diseases, but most of them are not very effective

Field rotation and the use of non-host plants or disease-resistant plants are the main management measures to control root-knot nematodes, but they have certain limitations. For example, most species of root-knot nematodes have a wide host range. Most of the population is a mixed population of several species, and the control effect is difficult to sustain

In addition, chemical nematicides such as methyl bromide, bisulphate, gramazan, carbofuran, abamectin, etc. have been widely used and achieved certain effects, but they are likely to cause soil environmental pollution, excessive residual toxicity of vegetables, etc., affecting quality even sell

Other measures such as sun exposure, flooding, use of disease-free seedlings, and soil improvement can achieve some control effects in some cases, but the effect is minimal or has relatively large limitations