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A method for increasing the yield of arginine

An arginine and seed technology, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of only 97% pass rate at one time, no guarantee of product quality, labor and time-consuming, etc. Achieve the effect of reducing energy consumption, reducing damage and improving the quality of filtrate

Inactive Publication Date: 2015-09-30
SHANDONG MINQIANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation liquid in the biological fermentation process contains a large amount of organic and inorganic impurities. The traditional post-extraction treatment process does not guarantee the quality of the product, and it is labor-intensive and time-consuming
The prior art uses plate and frame filtration during extraction, which takes a long time, low yield and large loss
The traditional process can only achieve a total yield of 50%, and the first pass rate is only 97%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0030] The preparation of fermented liquid comprises the following steps:

[0031] (1) Slant culture: insert the MQA121 strain into the sterilized slant medium (formulation glucose 2.5-4.0%, (NH 4 ) 2 SO 4 1.0-2.5%, KH 2 PO 4 0.3-0.8%, K 2 HPO 4 ·3H 2 O0.3-0.8%, MgSO 4 ·7H 2 O0.05-0.1%, FeSO 4 ·7H 2 O 0.002-0.004%, MnSO 4 ·H 2 O0.002-0.010%, agar powder 2.0%, pH6.4~7.0. ), cultivated at 32°C for 32 hours to obtain slant strains;

[0032] (2) Shake flask strains: put the cultured slant strains into the shake flask sterilized seed medium and cultivate them at 32°C for 22-32 hours to obtain the shake flask seed liquid;

[0033] (3) First-level seed culture: put the cultivated shake flask seed solution into the sterilized first-level seed medium, and cultivate it at 32°C for 22-28 hours to obtain the first-level seed solution;

[0034] (4) Secondary seed culture: insert the cultivated primary seed liquid into the sterilized secondary seed medium and cultivate at 32°...

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PUM

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Abstract

The invention discloses a method for improving the yield of arginine. According to the method, a ceramic membrane is adopted to replace the conventional sheet frame for filtration, so that the quality of filtrate is improved, the occupied area is reduced, the yield of a product is increased, and the labor intensity is alleviated. Due to the preparation method disclosed by the invention, the total yield of a finished product can reach 86.5 percent above relative to fermentation liquor (which is calculated relative to the net content of the arginine without thallus); the first-pass yield of the finished product is 99.8 percent.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a method for increasing the yield of arginine. Background technique [0002] Traditional biological fermentation process to prepare arginine. The fermentation liquid in the biological fermentation process contains a large amount of organic and inorganic impurities. The traditional post-extraction treatment process does not guarantee the quality of the product, and it is labor-intensive and time-consuming. The prior art adopts plate and frame filtration during extraction, which takes a long time, low yield and large loss. The traditional process can only achieve a total yield of 50%, and the first pass rate is only 97%. Contents of the invention [0003] The object of the present invention is to provide a method for improving the yield of arginine aiming at the defects mentioned above. The method adopts the ceramic membrane to replace the traditional plate and f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/10C07C279/14C07C277/08C12R1/13
Inventor 高法民高树营
Owner SHANDONG MINQIANG BIOTECH