Molecular identification method for China rana rugulosa and Thailand rana rugulosa

Abstract

In order to solve the identification problem of China rana rugulosa and Thailand rana rugulosa which have exactly-like appearance during actual protection of China rana rugulosa, the invention provides a molecular identification method for China rana rugulosa and Thailand rana rugulosa. The identification method is based on PCR technology, helps to realize rapid identification on China rana rugulosa and Thailand rana rugulosa by designing a specific primer for amplification, and specifically comprises the steps: 1) extracting DNA of a sample; 2) detecting sample DNA; 3) performing PCR amplification by employing four pairs of specific primers; and 4) performing identification on the specific amplified product. According to the method, by designing the four pairs of primers related to different gene positions, China rana rugulosa and Thailand rana rugulosa can be identified by PCR amplification, the primers are strong in specificity, the agarose gel electrophoresis characteristic spectral band is high in stability and has no regional difference, and the method can help to obtain reliable beneficial effect during practical application.

Description

technical field [0001] The invention relates to a molecular identification method of frogs, in particular to a molecular identification method of Chinese tiger frog and Thai tiger frog, and belongs to the field of biotechnology. Background technique [0002] Tiger frogs have rough skin with dark black markings on the head and sides. There are more than a dozen rows of longitudinally arranged skin ribs on the back, and brown-black small warts are scattered between the skin ribs. The ventral surface is white or gray, and there are also irregular markings, the pharynx and chest have gray-brown spots, and the front and rear limbs have horizontal spots. Due to human overfishing, the number of tiger frogs has been greatly reduced, and it is listed as a national second-level key protected animal, and it is also the only national-level protected animal among frogs. Along with the artificial introduction of the Thai tiger frog in the Thai farms, a large number of Thai tiger frogs a...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that the Chinese tiger frog and the Thai tiger frog are difficult to identify from the morphological characteristics, the present invention uses molecular biology technology to solve the molecular identification of the Chinese tiger frog and the Thai tiger frog, and provides a design based on PCR technology. Specific primers are used to amplify, so as to realize the method of rapid identification of tiger frogs in China and tiger frogs in Thailand

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