Quality detection method for fructus forsythiae leaf medicinal materials
A quality detection method, the technology of forsythia leaves, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problems of forsythia leaf medicine quality standard research, incomplete extraction of forsythiaside, poor separation, etc. To achieve the effect of promoting full development and utilization
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Embodiment 1
[0229] Embodiment 1: Identification of forsythia leaves
[0230] Take 1g of forsythia leaf powder, add 20ml of methanol, seal it up, ultrasonically treat it for 20 minutes, filter, evaporate the filtrate to dryness, add 20ml of water to dissolve the residue, filter the insoluble matter, collect the filtrate, and saturate it with 3 times the volume of ethyl acetate Shake and extract 3 times, combine the extracts, recover under reduced pressure, dissolve the residue with 5ml methanol, and use it as the test solution; take the forsythin reference substance, add methanol to make a solution containing 0.25mg per 1ml, and use it as the reference solution I Get Forsythiaside A reference substance, add methanol to make 1ml solution containing 0.2mg, as reference substance solution II; test according to thin-layer chromatography, draw the test solution, reference substance solution I, reference substance solution II each 3 μl, respectively spot on the same silica gel G thin-layer plate...
Embodiment 2
[0231] Embodiment 2: Identification of forsythia leaves
[0232] Take 0.3g of Forsythia leaf powder, add 25ml of methanol, seal it up, ultrasonicate for 25 minutes, filter, evaporate the filtrate to dryness, add 15ml of water to the residue to dissolve, filter the insoluble matter, collect the filtrate, shake it with 2 times the volume of water-saturated methanol Shake and extract 4 times, combine the extracts, recover under reduced pressure, dissolve the residue with 8ml of methanol, and use it as the test solution; take the forsythin reference substance, add methanol to make a solution containing 0.25mg per 1ml, and use it as the reference substance solution I; Take forsythiaside A reference substance, add methanol to make 1ml solution containing 0.2mg, as reference substance solution II; test according to thin-layer chromatography, absorb 2 μl each of the test solution, reference substance solution I, and reference substance solution II , were respectively spotted on the sa...
Embodiment 3
[0233] Embodiment 3: Identification of forsythia leaves
[0234] Take 1.8g of forsythia leaf powder, add 25ml of methanol, seal it up, ultrasonicate for 15 minutes, filter, evaporate the filtrate to dryness, add 30ml of water to dissolve the residue, filter the insoluble matter, collect the filtrate, and use 5 times the volume of water to saturate petroleum ether Shake and extract twice, combine the extracts, recover under reduced pressure, dissolve the residue with 4ml of methanol, and use it as the test solution; take the forsythin reference substance, add methanol to make a solution containing 0.25mg per 1ml, and use it as the reference solution I Get Forsythiaside A reference substance, add methanol to make 1ml solution containing 0.2mg, as reference substance solution II; test according to thin-layer chromatography, draw the test solution, reference substance solution I, reference substance solution II each 8 μl were spotted on the same silica gel G thin-layer plate, deve...
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