Method for detecting sensitivity of striped rice borers or pink rice borers to Bt-transgenic rice

Abstract

The invention discloses a method for detecting sensitivity of striped rice borers or pink rice borers to Bt-transgenic rice. The method includes the steps: taking Bt-transgenic rice seeds, soaking the Bt-transgenic rice seeds for 24-48h in distilled water at the temperature of 37 DEG C, moisturizing and sprouting the seeds after the seeds show white germs, and performing standby application when the germs grow to reach 0.8-1.5cm; selecting the rice germs without difference of Bt-poison protein expression quantities, and simultaneously feeding especially early larvae of the striped rice borers to be tested or the pink rice borers to be tested and especially early larvae of the striped rice borers fed indoors or the pink rice borers fed indoors by the aid of the rice germs; recording the number of live larvae after the especially early larvae are fed for 5-10 days, calculating the death rate, and comparing the especially early larvae of the striped rice borers to be tested or the pink rice borers to be tested with the especially early larvae of the striped rice borers fed indoors or the pink rice borers fed indoors. The method has the advantages of short test cycle, simplicity and convenience in operation, speediness and the like.

Description

technical field [0001] The invention relates to a method for detecting the susceptibility of rice stem borer or rice borer to transgenic Bt rice. Background technique [0002] Both Chilosia officinalis and Scutellaria officinalis belong to the order Lepidoptera, and are the most serious frequent pests on rice in my country. They are distributed in various rice regions in China, but they mainly occur in the Yangtze River Basin and the southern rice regions. In recent years, The number showed an obvious upward trend, posing a serious threat to agricultural production. [0003] Although traditional chemical control methods can effectively control insect pests in a short period of time, they pollute the environment, kill natural enemies, destroy ecological balance and increase production costs. Therefore, breeding insect-resistant rice is the most economical and effective method. Since the first Bt-transformed rice was successfully developed in 1988, different types of Bt insect...

AI Technical Summary

Problems solved by technology

[0002] Both Chilosia officinalis and Scutellaria officinalis belong to the order Lepidoptera, and are the most serious frequent pests on rice in my country. They are distributed in various rice regions in China, but they mainly occur in the Yangtze River Basin and the southern rice regions. In recent years, The number is on the rise, posing a serious threat to agricultural production

The traditional monitoring method is to feed borer pests with rice stalks transformed into Bt rice in the field, and evaluate the sensitivity of pests to Bt rice by statistical mortality. This method is cumbersome and time-consuming

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