Simple method for preparing nano silver from bacterial fermentation solution

A technology of bacterial fermentation and nano-silver, applied in the biological field, can solve the problems of relatively high preparation technology requirements, and achieve the effects of strong operability, simple and easy preparation method, and low cost

Active Publication Date: 2014-04-09
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many preparation methods for nano-silver particles, mainly chemical methods such as liquid-phase chemical reduction method, electrochemical method, and photochemical reduction method, and physical methods such as reduction ball milling method, evaporation condensation method, and atomization method. Common, but also need to strictly control the reaction conditions, the requirements for the preparation technology are relatively high

Method used

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  • Simple method for preparing nano silver from bacterial fermentation solution
  • Simple method for preparing nano silver from bacterial fermentation solution
  • Simple method for preparing nano silver from bacterial fermentation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Inoculate Staphylococcus aureus into 100ml of sterilized MH medium, mix thoroughly, place in a constant temperature water bath shaker at 37°C for 24 hours, add the culture solution to a centrifuge tube and centrifuge at 5000rpm for 5 minutes. The bacterial pellet was separated from the supernatant (bacterial suspension). Add 200 μl of 0.1M silver nitrate solution to 20ml of supernatant to make the concentration of silver ions 1mM, place on a water bath shaker at 37°C for 24 hours, and then ultrasonically crush the shaken solution in an ice bath, and output The power is 150 watts, ultrasound is 30s, interval is 30s, repeat 6 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 1 shown, from figure 1 It can be seen that the nano-silver solution in this embodiment is indeed a nano-silver solution.

Embodiment 2

[0017] Inoculate Staphylococcus aureus into 100ml of sterilized MH medium, mix thoroughly, place in a constant temperature water bath shaker at 37°C for 24 hours, add the culture solution to a centrifuge tube and centrifuge at 5000rpm for 5 minutes. The bacterial pellet was separated from the supernatant (bacterial suspension). Add 1ml of 0.1M silver nitrate solution to 20ml of the supernatant to make the concentration of silver ions 5mM, place it on a water bath shaker at 37°C for 24 hours, and then ultrasonically break the shaken solution under ice bath conditions, output The power is 200 watts, 30s of ultrasound, 30s of rest, repeated 6 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 2 shown, from figure 2 It can be seen that the nano-silver solution in this embodiment is indeed a nano-silver solution.

Embodiment 3

[0019] Inoculate Staphylococcus aureus into 100ml of sterilized MH medium, mix thoroughly, place in a constant temperature water bath shaker at 37°C for 24 hours, add the culture solution to a centrifuge tube and centrifuge at 5000rpm for 5 minutes. The bacterial pellet was separated from the supernatant (bacterial suspension). Add 2ml of 0.1M silver nitrate solution to 20ml of the supernatant to make the concentration of silver ions 10mM, place it on a water bath shaker at 37°C for 5h, and then ultrasonically crush the shaken solution in an ice bath, and output The power is 200 watts, 30s of ultrasound, 30s of rest, repeated 10 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: image 3 shown, from image 3 It can be seen that the nano-silver solution in this embodiment is indeed a nano-silver solution.

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Abstract

The invention discloses a simple method for preparing nano silver from a bacterial fermentation solution. The method comprises the steps of inoculating staphylococcus aureus or enterococcus faecalis to a Mueller-Hinton culture medium, implementing shake cultivation for 12-72 hours at 25-37 DEG C, separating the thallus from bacterium suspension, reserving the bacterium suspension, adding a silver nitrate solution to the bacterium suspension so that silver ion concentration is 0.5-10mM, implementing shake cultivation for 5-72 hours at 25-37 DEG C, carrying out ultrasonic treatment, filtering the solution after the ultrasonic treatment, and reserving filtrate to obtain a nano silver solution. The method, which is used for preparing nano silver particles through the bacterial fermentation solution, is simple and easy, strong in operability and low in cost, and is free from toxic and harmful substances to the environment, thus being a simple and environment-friendly nano silver preparation technology.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a simple method for preparing nano-silver by using bacterial fermentation liquid. Background technique: [0002] Silver nanoparticles (Ag-NPs for short) are known as a new generation of antibacterial agents in the 21st century, which have the advantages of broad-spectrum antibacterial properties, high-efficiency antibacterial activity, low toxicity and not easy to make microorganisms resistant to drugs. Nanosilver has been widely used in various medical materials, such as wound dressings, surgeon's masks, medical catheters, medical surgical instruments, and tissues implanted in the human body. Based on the excellent antibacterial performance of nano-silver, its application prospect is broad, and it has a wide range of market demands in the fields of daily necessities, medical and health care, building materials, and electronics industry. [0003] There are many preparat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/445C12R1/46
Inventor 李文茹施庆珊梁青谢小保陈仪本
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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