Method for preparing nano-silver by means of microalgae
A nano-silver and micro-algae technology, applied in the biological field, can solve the problems of relatively high preparation technology requirements, and achieve the effects of strong operability, simple and easy preparation method, and low cost
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Embodiment 1
[0012] Add the cultured chlorella algae liquid to a centrifuge tube and place it on a centrifuge at 5000rpm for 5 minutes, resuspend 1g of microalgae with a wet weight in 20mL of deionized water, and ultrasonically break the algae cells under ice bath conditions, and the ultrasonic output The power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 5 times. Centrifuge to remove the algae precipitate, add silver nitrate solution to 20ml supernatant to make the final concentration of silver ions 10μg / mL, place on a water bath shaker at 30°C for 5h, and then ultrasonicate the reaction solution in an ice bath For crushing, the output power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 5 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 1 shown, from figur...
Embodiment 2
[0014] Add the cultured chlorella algae liquid to a centrifuge tube and place it on a centrifuge at 5000rpm for 5 minutes, resuspend the algae cell pellet with a wet weight of 2g in 20ml of sterilized deionized water, and ultrasonically break the algae in an ice bath For cells, the output power was 200 watts, sonication was performed for 1 min, with an interval of 1 min, and was repeated 10 times. Centrifuge to remove the algae precipitate, add silver nitrate solution to 20ml supernatant to make the final concentration of silver ions 20μg / mL, place on a water bath shaker at 40°C for 5h, and then ultrasonicate the reaction solution in an ice bath For crushing, the output power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 3 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 2 shown, from ...
Embodiment 3
[0016] Add the cultured chlorella algae solution to a centrifuge tube and centrifuge at 5000rpm for 5 minutes, resuspend the algae cell pellet with a wet weight of 5g in 20mL sterilized deionized water, and ultrasonically break the algae in an ice bath. For cells, the output power is 200 watts, ultrasonication is performed for 1 min, and the interval is 1 min, repeated 5 times. Centrifuge to remove the algal precipitate, add silver nitrate solution to 20ml supernatant to make the concentration of silver ions 50μg / mL, place on a water bath shaker at 20°C for 5h, and then ultrasonically break the reaction solution in an ice bath , with an output power of 150 watts, ultrasonication for 1 min, with an interval of 1 min, and repeated 10 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: image 3 shown, from image 3 It can be seen that t...
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