Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing nano-silver by means of microalgae

A nano-silver and micro-algae technology, applied in the biological field, can solve the problems of relatively high preparation technology requirements, and achieve the effects of strong operability, simple and easy preparation method, and low cost

Active Publication Date: 2015-04-22
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many preparation methods for nano-silver particles, mainly chemical methods such as liquid-phase chemical reduction method, electrochemical method, and photochemical reduction method, and physical methods such as reduction ball milling method, evaporation condensation method, and atomization method. Common, but also need to strictly control the reaction conditions, the requirements for the preparation technology are relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing nano-silver by means of microalgae
  • Method for preparing nano-silver by means of microalgae
  • Method for preparing nano-silver by means of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Add the cultured chlorella algae liquid to a centrifuge tube and place it on a centrifuge at 5000rpm for 5 minutes, resuspend 1g of microalgae with a wet weight in 20mL of deionized water, and ultrasonically break the algae cells under ice bath conditions, and the ultrasonic output The power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 5 times. Centrifuge to remove the algae precipitate, add silver nitrate solution to 20ml supernatant to make the final concentration of silver ions 10μg / mL, place on a water bath shaker at 30°C for 5h, and then ultrasonicate the reaction solution in an ice bath For crushing, the output power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 5 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 1 shown, from figur...

Embodiment 2

[0014] Add the cultured chlorella algae liquid to a centrifuge tube and place it on a centrifuge at 5000rpm for 5 minutes, resuspend the algae cell pellet with a wet weight of 2g in 20ml of sterilized deionized water, and ultrasonically break the algae in an ice bath For cells, the output power was 200 watts, sonication was performed for 1 min, with an interval of 1 min, and was repeated 10 times. Centrifuge to remove the algae precipitate, add silver nitrate solution to 20ml supernatant to make the final concentration of silver ions 20μg / mL, place on a water bath shaker at 40°C for 5h, and then ultrasonicate the reaction solution in an ice bath For crushing, the output power is 150 watts, ultrasound is performed for 1 min, and the interval is 1 min, repeated 3 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: figure 2 shown, from ...

Embodiment 3

[0016] Add the cultured chlorella algae solution to a centrifuge tube and centrifuge at 5000rpm for 5 minutes, resuspend the algae cell pellet with a wet weight of 5g in 20mL sterilized deionized water, and ultrasonically break the algae in an ice bath. For cells, the output power is 200 watts, ultrasonication is performed for 1 min, and the interval is 1 min, repeated 5 times. Centrifuge to remove the algal precipitate, add silver nitrate solution to 20ml supernatant to make the concentration of silver ions 50μg / mL, place on a water bath shaker at 20°C for 5h, and then ultrasonically break the reaction solution in an ice bath , with an output power of 150 watts, ultrasonication for 1 min, with an interval of 1 min, and repeated 10 times. The ultrasonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano-silver solution. The electron microscope picture of this nano-silver solution is as follows: image 3 shown, from image 3 It can be seen that t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a simple method for preparing nano-silver by means of microalgae. According to the simple method for preparing nano-silver by means of microalgae, a solution of chlorella or limnothrix or scenedesmus quaclricauda is centrifuged, algal cells are deposited and re-suspended in sterilized deionized water, ice-bathing and breaking are conducted by placing the sterilized deionized water containing the algal cells under ultrasonic waves, breaking is conducted for three to ten minutes under the condition that the output power ranges from 150 W to 200 W, centrifugation is conducted, algal sediment is removed, silver nitrate is added to the algal solution, the concentration of silver ions ranges from 5 microgram / mL to 100 microgram / mL, the oscillation reaction is conducted for one to five hours by placing the mixture on a water bath shaker at the temperature of 20-40 DEG C, and then a nano-silver solution is obtained by conducting ultrasonic treatment and membrane filtering. According to the method, nano-silver particles are prepared by means of microalgae, the preparation method is simple, easy to implement, high in operability and low in cost, no toxic and harmful substance is discharged into the environment during preparation, and the method is a simple and environmental-friendly technique for preparing nano-silver.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing nano-silver by using microalgae. Background technique: [0002] As early as in ancient times, metallic silver has been recognized as antibacterial and bactericidal, and has broad-spectrum antibacterial activity. In recent years, the new progress in the research of metal nanoparticles has promoted the application of nano-silver in the field of antibacterial. Studies have found that nano-silver prepared by various methods has efficient antibacterial activity. Nano-silver is known as a new generation of antibacterial agents in the 21st century. It has broad-spectrum and high-efficiency antibacterial properties. Nano-silver has been widely used as an antibacterial material, such as burn dressings, operating tables, water purification devices and Some medical equipment etc. Based on the excellent antibacterial performance of nano-silver, its applicati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B22F9/24
Inventor 李文茹梁青施庆珊冯静谢小保黄小茉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com