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A Simple Method for Preparation of Nano Silver Using Bacterial Fermentation Broth

A technology of bacterial fermentation and nano-silver, applied in the biological field, can solve the problem of relatively high requirements for preparation technology, and achieve the effects of strong operability, simple and easy preparation method and low cost.

Active Publication Date: 2015-09-09
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many preparation methods for nano-silver particles, mainly chemical methods such as liquid-phase chemical reduction method, electrochemical method, and photochemical reduction method, and physical methods such as reduction ball milling method, evaporation condensation method, and atomization method. Common, but also need to strictly control the reaction conditions, the requirements for the preparation technology are relatively high

Method used

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  • A Simple Method for Preparation of Nano Silver Using Bacterial Fermentation Broth
  • A Simple Method for Preparation of Nano Silver Using Bacterial Fermentation Broth
  • A Simple Method for Preparation of Nano Silver Using Bacterial Fermentation Broth

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Inoculate Staphylococcus aureus into 100ml sterile MH medium, mix well and place it in a constant temperature water bath shaker at 37°C for 24 hours with shaking, add the culture solution to a centrifuge tube and place it on a centrifuge at 5000 rpm for 5 minutes. The bacterial pellet is separated from the supernatant (bacterial suspension). Add 200μl of 0.1M silver nitrate solution to 20ml of supernatant to make the concentration of silver ions 1mM. Place it on a water bath shaker at 37°C and shake for 24h. Then the shaken solution is ultrasonically broken under ice bath conditions. The power is 150 watts, the ultrasound is 30 seconds, the interval is 30 seconds, and it is repeated 6 times. The sonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano silver solution. The electron micrograph of the nano silver solution is as follows figure 1 Shown from figure 1 It can be seen that the nano silver solution of this embodiment is indeed a nan...

Embodiment 2

[0017] Inoculate Staphylococcus aureus into 100ml sterile MH medium, mix well and place it in a constant temperature water bath shaker at 37°C for 24 hours with shaking, add the culture solution to a centrifuge tube and place it on a centrifuge at 5000 rpm for 5 minutes. The bacterial pellet is separated from the supernatant (bacterial suspension). Add 1ml of 0.1M silver nitrate solution to 20ml of supernatant to make the concentration of silver ions 5mM, place it on a water bath shaker at 37°C and shake for 24 hours, then the shaken solution is ultrasonically broken under ice bath conditions, and output The power is 200 watts, the ultrasound is 30 seconds, the interval is 30 seconds, and it is repeated 6 times. The sonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano silver solution. The electron micrograph of the nano silver solution is as follows figure 2 Shown from figure 2 It can be seen that the nano silver solution of this embodiment i...

Embodiment 3

[0019] Inoculate Staphylococcus aureus into 100ml sterile MH medium, mix well and place it in a constant temperature water bath shaker at 37°C for 24 hours with shaking, add the culture solution to a centrifuge tube and place it on a centrifuge at 5000 rpm for 5 minutes. The bacterial pellet is separated from the supernatant (bacterial suspension). Add 2ml of 0.1M silver nitrate solution to 20ml of supernatant to make the concentration of silver ions 10mM, place it on a water bath shaker at 37℃ and shake for 5h, then the shaken solution is ultrasonically broken under ice bath conditions, and output The power is 200 watts, the ultrasound is 30 seconds, the interval is 30 seconds, and it is repeated 10 times. The sonicated solution was filtered with a 0.22 μm filter membrane to obtain a clear nano silver solution. The electron micrograph of the nano silver solution is as follows image 3 Shown from image 3 It can be seen that the nano silver solution of this embodiment is indee...

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Abstract

The invention discloses a simple method for preparing nano silver from a bacterial fermentation solution. The method comprises the steps of inoculating staphylococcus aureus or enterococcus faecalis to a Mueller-Hinton culture medium, implementing shake cultivation for 12-72 hours at 25-37 DEG C, separating the thallus from bacterium suspension, reserving the bacterium suspension, adding a silver nitrate solution to the bacterium suspension so that silver ion concentration is 0.5-10mM, implementing shake cultivation for 5-72 hours at 25-37 DEG C, carrying out ultrasonic treatment, filtering the solution after the ultrasonic treatment, and reserving filtrate to obtain a nano silver solution. The method, which is used for preparing nano silver particles through the bacterial fermentation solution, is simple and easy, strong in operability and low in cost, and is free from toxic and harmful substances to the environment, thus being a simple and environment-friendly nano silver preparation technology.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and specifically relates to a simple method for preparing nano silver by using bacterial fermentation broth. Background technique: [0002] Silver nanoparticles (Ag-NPs) are known as the new generation of antibacterial agents in the 21st century. They have the advantages of broad-spectrum antibacterial properties, high antibacterial activity, low toxicity and resistance to microorganisms. Nano silver has been widely used in various medical materials, such as wound dressings, surgeons' masks, medical catheters, medical surgical instruments, and tissues implanted in the human body. Based on the excellent antibacterial properties of nano-silver, its application prospects are broad, and there are broad market demands in the fields of daily necessities, medical and health, building materials, and electronics industries. [0003] There are many methods for preparing silver nanoparticles, mainly chemical methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/445C12R1/46
Inventor 李文茹施庆珊梁青谢小保陈仪本
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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