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A special enzyme for growing pigs added with cellulase and its preparation method

A technology of cellulase and special enzymes, applied in biochemical equipment and methods, additional food elements, enzymes, etc., can solve problems such as high feeding costs, slow growth of livestock and poultry, unreasonable enzyme ratios, etc., and achieve good stability Sexuality and preservation of activity, promote digestion and absorption, the effect of a reasonable ratio

Active Publication Date: 2016-09-21
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many kinds of compound enzymes for feed on the market, but some enzymes have unreasonable ratios and fail to achieve the desired effect, resulting in slow growth and low productivity of livestock and poultry; some enzymes have low activity and large dosage, resulting in Feeding costs are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0050] Preferably, a special enzyme for growing pigs is composed of the following parts by weight: 1 part of astragalus powder, 30 parts of xylanase, 6 parts of protease, 4 parts of pectinase, cellulose 10 parts of enzyme, 15 parts of β-glucanase and its culture, 5 parts of mesophilic α-amylase.

Embodiment 3

[0051] Embodiment 3: consists of the following components in parts by weight: 2 parts of Chinese herbal medicine powder, 40 parts of xylanase, 5 parts of protease, 2 parts of pectinase, 16 parts of cellulase, β-glucanase and its 20 parts of culture, 5 parts of mesophilic α-amylase. The Chinese herbal medicine described in the Chinese herbal medicine powder consists of astragalus and codonopsis pilosula, the mass ratio of astragalus and codonopsis pilosula is 1:1, and they can be pulverized before use.

[0052] Embodiment 4 effect comparative experiment

[0053] A total of 120 growing pigs were selected and divided into experimental group and control group according to no difference in body weight, pedigree and sex. Each group had 3 replicates, and each replicate had 20 pigs. Experimental period is 28 days, and every ton of feed of experimental group and matched group all adds compound enzyme preparation 120g, and the compound enzyme preparation used in experimental group is t...

Embodiment 5

[0055] The influence of embodiment 5 Chinese herbal medicine powder on enzyme preservation activity

[0056] The special enzyme for growing pigs obtained by the preparation method described in Example 3 is sample 1, and the special enzyme for growing pigs obtained by removing all the Chinese herbal medicine powder preparation methods in Example 3 is sample 2, and the preservation activity of the two samples is compared . The above samples were stored at 45°C for 18 hours, and then the enzyme activity was measured. The results showed that the residual enzyme activity of sample 1 was above 75%, and the residual enzyme activity of sample 2 remained at about 60%.

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Abstract

The invention relates to a special enzyme for growing pigs and a preparation method thereof, belonging to the field of feeding enzymes. The special enzyme for growing pigs comprises the following components in parts by weight: 0.5-2 parts of Chinese herbal medicine powder, 20-40 parts of xylanase, 5-8 parts of protease, 2-5 parts of pectinase, 8-16 parts of cellulase, 10-20 parts of beta-glucanase, and 2-6 parts of intermediate temperature alpha-amylase, wherein the production strain of cellulase is trichoderma reesei 601-17, and the preservation number is CCTCC (China Center For Type Culture Collection) M2013540. The preparation method comprises steps of cultivating slope strains so as to obtain liquid seeds; fermenting by a method combining low temperature and step-by-step inoculation; and centrifuging the fermentation liquor, collecting, concentrating, filtering, drying so as to obtain the cellulase. After being concentrated and dried, the cellulase further improves the unit activity and vitality, effectively supplements insufficient endogenous enzyme, promotes digestive absorption of nutrient substances, and improves the use ratio of fodder.

Description

technical field [0001] The invention belongs to the field of enzyme preparations, and in particular relates to a composite enzyme and a manufacturing method thereof. Background technique [0002] Scientists from the former Soviet Union added starch dextrinase to the diet of early weaned piglets, which increased daily weight gain by 14.6% and carbohydrate digestibility by 77.2%. According to relevant reports in the United States, Collier supplemented the diet of early-weaned piglets with enzyme preparations (including protease, amylase, and dextranase). As a result, the daily gain of the experimental group was 25% higher than that of the control group, and the feed conversion rate was increased by 15.5%. %. Scholars in West Germany have conducted many experimental studies on adding compound enzyme preparations to fattening pigs. Adding 0.1% compound enzyme preparations to barley-based diets, the results are: the growth rate of the experimental group increased by 5.1% compare...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/88C12N9/26C12N9/18C12N9/50A23K20/189A23K20/00
CPCA23K20/00A23K20/189A23K50/30C12N9/18C12N9/2437C12N9/244C12N9/248C12N9/50C12N9/88
Inventor 李洪兵朱永明易继云刘文明向左东
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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