A method for quantitative detection of Banana Fusarium wilt Tropical Race 4 from soil

Abstract

The present invention discloses a method for quantitatively detecting Fusarium oxysporium f.sp.cubense Tripical race 4 from soil. The method comprises: carrying out a pre-treatment on a sample requiring detection, and extracting DNA of the sample requiring detection; preparing specific primers; carrying out a fluorescent nucleic acid isothermal amplification detection technology RealAmp reaction; constructing a RealAmp standard curve; and judging results. The method has the following characteristics that: defects of the gene amplification method in the prior art are overcome, characteristics of high specificity, high sensitivity, quantitative detection lower limitation of 4.3*10<-4> ng / mul, easy operation, short time consumption (only requires 2-3 h), pollutant influence resistance, on site soil sample detection, no requirement of expensive and precise temperature cycling equipment, and only requirement of thermostatic equipment capable of achieving a temperature of 63 DEG C are provided, the reaction product analysis judgment method is simple and only requires observation by naked eye, the Fusarium oxysporium f.sp.cubense Tripical race 4 content in the soil can be quantitatively determined, and the method is suitable for wide promotion application.

Description

technical field [0001] The invention belongs to the detection field of the banana fusarium wilt fungus Tropical race No. 4, and in particular relates to a method for quantitatively detecting the banana Fusarium wilt fungus Tropical race No. 4 from soil. Background technique [0002] Banana is a plant of the genus Musa of the Musaceae family, and is an important economic crop and food crop in tropical and subtropical regions. Banana Fusarium Wilt is a devastating disease of the banana industry, which seriously restricts the healthy development of my country's banana industry. [0003] The pathogen of banana wilt is Fusariumoxysporum Schlevht.f.sp.cubense (E.F.Smith) Snyd.&Hans., formerly known as FusariumcubenseE.F.Smith. Fusarium sporogenes Cuban specialization. The fungus is divided into 3 races. In our country, it is mainly the No. 1 race and the No. 4 race. Race 1 has a long history, and the harm of race 1 can be solved by replanting Cavendish bananas. Race No. 4 was...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a method for quantitatively detecting the banana fusarium wilt pathogen tropical race No. 4 from the soil, aiming to solve the problem that the traditional PCR detection technology needs to extract relatively high-purity sample genomic DNA, takes a long time, and cannot detect soil samples on site , cannot quantitatively detect pathogenic bacteria from the soil, and requires expensive and sophisticated temperature cycle devices, high cost, and difficult to operate

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