Detection method for measuring content of thiophanate methyl

A technology for the detection of thiophanate-methyl and its detection method, which is applied in the detection field of the determination of thiophanate-methyl residues in bean sprouts, and can solve the problems that the analysis is easily affected by environmental changes and the detection steps are cumbersome

Inactive Publication Date: 2014-04-23
上海德诺产品检测有限公司
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  • Abstract
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Problems solved by technology

[0004] The technical problem solved by the present invention is to overcome the defects of existing detection methods for measuring thiophanate-methyl, which are easily affected by environmental changes and complicated detection steps, and provide a detection method for determining the content of thiophanate-methyl. The method is especially suitable for the determination of thiophanate-methyl residues in sprout plants, which has high detection accuracy, is less affected by environmental changes, and can quickly perform qualitative and quantitative determinations of thiophanate-methyl

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  • Detection method for measuring content of thiophanate methyl
  • Detection method for measuring content of thiophanate methyl
  • Detection method for measuring content of thiophanate methyl

Examples

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Embodiment 1

[0072] Detection of thiophanate-methyl content in soybean sprouts:

[0073] The detection method of measuring thiophanate-methyl content, it may further comprise the steps:

[0074] (1) Weigh 5.15g of the crushed soybean sprout sample, put it in a 50mL polypropylene centrifuge tube, add 10mL of acidified methanol aqueous solution, homogenize with a homogenizer for 2min, and centrifuge at 8000 rpm for 3min at 4°C , to obtain supernatant A and precipitate;

[0075] (2) Mix the precipitate with 10 mL of acidified methanol aqueous solution, homogenize with a homogenizer for 2 min, and centrifuge at 8,000 rpm for 3 min at 4°C to obtain supernatant B; combine supernatant A and supernatant B, dilute to obtain extract in 25mL volumetric flask;

[0076] (3) Vigorously shake 5mL of extract and 5mL of petroleum ether for 2min, centrifuge at 5000rpm at 4°C for 3min, remove the lower layer and filter through a filter membrane with a pore size of 0.22μm to obtain the sample solution;

[...

Embodiment 2

[0080] Detection of thiophanate-methyl content in mung bean sprouts:

[0081] The detection method of measuring thiophanate-methyl content, it may further comprise the steps:

[0082] (1) Weigh 5.05g of the crushed soybean sprout sample, put it in a 50mL polypropylene centrifuge tube, add 5.5mL of acidified methanol aqueous solution, homogenize with a homogenizer for 2min, and centrifuge at 5000 rpm at 3°C 7min to obtain supernatant A and precipitate;

[0083] (2) Mix the precipitate with 10 mL of acidified methanol aqueous solution, homogenize with a homogenizer for 2 min, and centrifuge at 6 °C for 7 min at a speed of 9000 rpm to obtain supernatant B; combine supernatant A and supernatant B, dilute to obtain extract in 25mL volumetric flask;

[0084] (3) Vigorously shake 5mL of extract and 5mL of petroleum ether for 2min, centrifuge at 6°C at 9000rpm for 1min, remove the lower layer and filter through a filter membrane with a pore size of 0.22μm to obtain the sample soluti...

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Abstract

The invention discloses a detection method for measuring the content of thiophanate methyl. The method comprises the following steps: (1) mixing a sample with an acidified methanol aqueous solution in the ratio of (5g-10g):10mL, and performing homogenization and centrifugation to obtain supernatant A and precipitates; (2) mixing the precipitates with the acidified methanol aqueous solution, performing homogenization and centrifugation to obtain supernatant B, mixing the supernatant A with the supernatant B, and making up to constant volume to obtain an extract; (3) mixing the extract with petroleum ether in the volume ratio of 1:1 to 1:3, performing oscillation and centrifugation, extracting a lower-layer solution, and performing filtration to obtain a sample solution; and (4) performing liquid chromatography-tandem mass spectrometry detection on the sample solution. The detection method is particularly applied to the measurement of residues of thiophanate methyl in bean spout plants, is high in detection accuracy, is slightly influenced by environmental changes, and can be used for performing qualitative and quantitative measurement on the thiophanate methyl rapidly.

Description

technical field [0001] The invention relates to a detection method for determining the content of thiophanate-methyl, in particular to a detection method for determining the residual amount of thiophanate-methyl in bean sprouts. Background technique [0002] Thioprazine-methyl is a broad-spectrum systemic and low-toxic fungicide with systemic, preventive and therapeutic effects. Can effectively prevent and control diseases of various crops, such as wheat, rice, sweet potato, melon, tomato, mulberry, apple tree, cereal, rape, cotton, sugar beet, potato, grape, tobacco, citrus tree, bamboo, peanut , orchids, etc. to prevent and control various diseases. According to its chemical structure, thiophanate-methyl is a substituted benzene fungicide. After being absorbed by plants, thiophanate-methyl is transformed into carbendazim. It mainly interferes with the formation of fungal hyphae, affects the division of germ cells, poisons the cell wall, and spores germinate and grow. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孟瑾郑小平韩奕奕何亚斌楼飞施雅马颖清
Owner 上海德诺产品检测有限公司
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