Rapid screening method for illegally added chemicals in small collaterals-activating pills

Abstract

The invention discloses a rapid screening method for illegally added chemicals in small collaterals-activating pills. The rapid screening method comprises the following steps: firstly, preparing a reference solution and a test solution; then, respectively absorbing the reference solution and the test solution, dropping the reference solution and the test solution on the same silica gel thin-layer plate, upstream developing the reference solution and the test solution by using a developing agent, taking the silica gel thin-layer plate out, drying the silica gel thin-layer plate, and respectively placing the silica gel thin-layer plate under an ultraviolet light lamp to observe the thin-layer chromatography; next, spraying a sulfuric acid and ethanol solution, placing the silica gel thin-layer plate in a baking oven and heating at a constant temperature until spots on the thin-layer plate are developed clearly; finally, respectively observing the thin-layer chromatography in sunlight and under the ultraviolet light lamp, if fluorescence quenching spots, fluorescent spots or spots with the same color appear at the same shift value as that of the reference solution, determining the sample result to be positive. Obviously, the rapid screening method is operated conveniently and rapidly and is low in cost; the problem of false positive interference in the rapid screening method is solved; high cost and plenty of time generated by the conventional determination by adopting infrared spectroscopy, high performance liquid chromatography-mass spectrometry (HPLC-MS) and the like are also avoided, so that whether the chemicals added illegally to the small collaterals-activating pills is possibly monitored effectively.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a rapid screening method for illegally added chemicals in Xiaohuoluo pills. Background technique [0002] Xiaohuoluo Pills is a traditional Chinese medicine preparation composed of 6 Chinese medicine decoction pieces including Dannanxing, Zhichuanwu, Zhicaowu, Dilong, frankincense (made) and myrrh (made). The main effects are dispelling wind and cold, resolving phlegm and dehumidification, promoting blood circulation and relieving pain. It is used for arthralgia caused by wind-cold-damp pathogen blockage and phlegm-stasis blockage of collaterals. Symptoms include limb joint pain, or cold pain, or tingling pain, or pain that is worse at night, joint flexion and extension difficulties, numbness and spasm. Its preparation method is to grind Dannanxing, Zhichuanwu, Zhicaowu, Dilong, frankincense (made), and myrrh (made) into fine powders, sieve, mix ...

AI Technical Summary

Problems solved by technology

However, because infrared spectrometers, HPLC-MS, and GC-MS instruments are expensive and must be operated by professional technicians, the maintenance costs are also extremely high, and rapid screening cannot be achieved to achieve the purpose of monitoring

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