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Micro-organism threat detection

A microbiological detection and microbiological technology, applied in the field of microbiological results to infer threats, can solve problems such as high labor costs

Inactive Publication Date: 2014-04-30
ZYZEBA TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0110] Problems remain in tracking the presence of microorganisms over an extended area, detecting the population's progress in terms of area and level of occurrence, and predicting future populations
[0111] Furthermore, if a population or nascent population is to be monitored over an extended natural area, the labor required to do so is prohibitively expensive, involving repeated measurements at many locations

Method used

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Examples

Experimental program
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Effect test

example 1

[0442] Example 1 - coliform bacteria

[0443] A preferred growth medium composition contains an indicator (e.g., resazurin), an inhibitor (e.g., bile salts) of non-specific microorganisms (i.e., those not tested), a protein source (e.g., casein-casein trypsin) digesta), an energy source (such as lactose), and at least one salt (such as NaCl). Examples of such compositions are described below; Table 1. In addition, percentage ranges for each component of the composition have been given within which coliform detection can still be performed according to the present invention. Another growth medium composition suitable for testing for the presence of coliform bacteria, including E. coli, is given in Table 2.

[0444] Table 1

[0445]

[0446]*Resazurin is a redox dye indicator – other similar indicators can be used – such as triphenyltetrazolium chloride (TCC) and rhombonic acid (Table 3)

[0447] *Resazurin is also known as (7-hydroxy-3H-phenoxazin-3-one 10-oxide) sodiu...

Embodiment 2

[0451] Example 2 - Staphylococcus

[0452] Examples of ranges of components in growth medium compositions that may be used to support growth and detect staphylococci are given in Table 3 below.

[0453] table 3

[0454]

[0455] It should be noted that the components of the compositions of Table 1, 2 or 3 may vary depending on the type of test to be performed. In the case of meat samples to be tested, the composition of Table 1 is preferably free of salt. Additionally, alternative redox dyes or other indicators may be utilized. Additionally, the protein, salt, and energy sources of the compositions described herein can be varied to support the growth of different alternative microorganisms.

[0456] Calibration plot showing growth (and death) patterns of coliform bacteria, including specific Escherichia coli species, grown in vials or other containers containing a standard growth medium (in this sense, a standard means a set of Containers with or without mesocoliforms...

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PUM

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Abstract

A method of tracking and predicting the spread of micro-organisms in an environment relies on the optical detection of the growth of micro-organisms within an analyte containing a growth medium for the micro-organism(s) to be detected. Standardization of the containers and growth medium for each micro-organism allows rapid detection of the growth pattern of particular organisms and the implementation of a regime of remote self-reporting of results from portable incubator units (1201) in the field which include a memory (1206) to store the results, a GPS unit (1204) to provide location information and a transmitter (1203) to send the results to a central plotting organization allowing rapid tracking and prediction of the spread of environmentally borne organisms.

Description

technical field [0001] The present invention generally relates to inferring threats from collating results of microbes detected across an extended area, to culturing and analyzing microbes in the laboratory and in the field to determine threats and collating results from multiple analysis instances to allow inferences to be made. [0002] More specifically, the invention relates to detecting and enumerating a plurality of different microorganisms from changes in the color of culture medium in which organisms are submerged, tracking populations of these microorganisms at certain locations and tracking the emergence and spread or decline of spatial populations of microorganisms. [0003] Additionally, the present invention relates to the standardization of growth media and auxiliaries for microorganisms in an analyte, as well as media to render such analyte biologically inert after analysis. [0004] More specifically, the present invention relates to providing a portable resett...

Claims

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Application Information

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IPC IPC(8): G01N21/00G01N1/10
CPCC12Q1/04G01N2001/021G01N2021/4726G01N2001/022G01N1/00C12Q1/10G01N21/51G01N21/25G01N21/272
Inventor 罗斯玛丽·凯瑟琳·卡梅伦·莎平
Owner ZYZEBA TESTING